| 盐酸沃尼妙林预混剂在猪组织中残留消除规律研究 |
| |
| 引用本文： | 张盈盈,陈小秋,李朋朋,陈翠兰,王铭宏,王春梅,李晶,齐元花,易维学,操继跃.盐酸沃尼妙林预混剂在猪组织中残留消除规律研究[J].兽医大学学报,2012(2):272-276. |
| |
| 作者姓名： | 张盈盈 陈小秋 李朋朋 陈翠兰 王铭宏 王春梅 李晶 齐元花 易维学 操继跃 |
| |
| 作者单位： | [1]华中农业大学动物医学学院药理与毒理实验室,湖北武汉430070 [2]天津市兽药饲料监察所,天津300402 [3]湖北龙翔药业有限公司,湖北武汉435402 |
| |
| 摘 要： | 建立了猪肝脏、肾脏、肌肉和脂肪中盐酸沃尼妙林的高效液相色谱检测方法并研究盐酸沃尼妙林预混剂在猪体内各组织中的残留消除规律。对24头健康猪以200mg/kg的剂量混饲给药21d。在停药后0、6、12、18、24、36h分别宰杀4头猪,采集各组织进行药物残留测定。方法的检测限为0.025～0.062 5μg/g,定量限为0.05～0.1μg/g,肝脏的平均回收率为75.5%～76.4%,变异系数为2.3%～3.8%;肾脏的平均回收率为75.8%～78.5%,变异系数为4.1%～6.0%;肌肉的平均回收率为79.3%～80.0%,变异系数为3.0%～4.7%;脂肪的平均回收率为76.7%～77.3%,变异系数为3.3%～5.4%。结果表明,盐酸沃尼妙林在肝脏中残留量最高,肾脏其次;肌肉和脂肪中的残留量显著低于肝脏和肾脏,停药24h时,残留量低于定量限;停药36h时残留量均低至检测限以下。盐酸沃尼妙林预混剂在猪组织中消除迅速,建议休药期为2d。
|
| 关 键 词： | 盐酸沃尼妙林 高效液相色谱 猪组织 残留 消除 |
Studies on residues depletion of hydrochloride valnemulin premix in the porcine tissues |
| |
| Authors: | ZHANG Ying-ying CHEN Xiao-qiu LI Peng-peng CHEN Cui-lan WANG Ming-hong WANG Chun-mei LI Jing QI Yuan-hua YI Wei-xue CAO Ji-yue |
| |
| Institution: | 1.Department of Pharmacology and Toxicology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;2.Tianjin Institute of Veterinary Drugs Control,Tianjin 300402,China;3.Hubei Longxiang Pharmaceutical Co.Ltd,Wuhan 435402,China) |
| |
| Abstract: | To develop high performance liquid chromatography（HPLC） method of valnemulin hydrochloric residue in porcine tissues,and to explore elimination rule on its residues in the swine tissues.Twenty-four healthy pigs were administered valnemulin hydrochloric at a dosage of 200 mg/kg for 21 d.Four pigs were slaughtered at 0,6,12,18,24,36 h after the last feeding,respectively.Tissues including kidney,liver,muscle and fat of each pig were collected,and valnemulin of tissues were determined by high performance liquid chromatography（HPLC）.The limit of detection（LOD） was 0.025-0.062 5 μg/g and the limit of quantification（LOQ） was 0.05-0.1 μg/g.The average recovery rate in liver was 75.5%-76.4% with coefficients of variation（CV） of 2.3%-3.8%;in kidney was 75.8%-78.5% with CV of 4.1%-6.0%;in muscle was 79.3%-80.0% with CV of 3.0%-4.7%;and in fat was 76.7%-77.3% with CV of 3.3%-5.4%.Results showed that the residues of valnemulin hydrochloric in liver was the highest,and lower in kidney.The residues of valnemulin hydrochloric in muscle and fat were much lower than that in liver and kidney,and was declined below the LOQ at 24 h,and dropped to LOD at 36 h of withdrawal period.The depletion of drugs in porcine tissues was fast.The withdrawal periods for all porcine tissues were 2. |
| |
| Keywords: | valnemulin hydrochloric HPLC porcine tissues residue depletion |
| 本文献已被 维普 等数据库收录！ |
|
