| 饲料中鸡源性成分PCR-DHPLC检测方法的建立 |
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| 引用本文： | 王金玲,贾金生,李俊环,张莹,王芳,高铁军.饲料中鸡源性成分PCR-DHPLC检测方法的建立[J].兽医大学学报,2012(3):493-496. |
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| 作者姓名： | 王金玲 贾金生 李俊环 张莹 王芳 高铁军 |
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| 作者单位： | [1]沈阳出入境检验检疫局,辽宁沈阳110016 [2]锦州市义县动物卫生监督所,辽宁锦州121100 |
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| 基金项目： | 沈阳市大型科学仪器设备共享专项资助项目（2007共享012） |
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| 摘 要： | 以鸡组织为材料,提取鸡组织的线粒体DNA,以鸡12SrRNA基因为靶基因设计引物,PCR反应扩增片段230bp;用13种畜禽和水产动物线粒体DNA验证该PCR引物的特异性,结果只有鸡线粒体DNA为阳性;PCR-DHPLC检测灵敏度在DNA水平上达到了2.04mg/L;在随机采集的22份饲料样品中,检出8份鸡源性成分样品。结果表明,本研究建立的PCR-DHPLC方法可特异、灵敏地对饲料中的鸡源性成分进行快速准确检测。
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| 关 键 词： | 饲料 鸡源性成分 PCR-DHPLC |
Establishment of PCR-DHPLC assay for detection of chicken tissue in feedstuff |
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| Authors: | WANG Jin-ling JIA Jin-sheng LI Jun-huan ZHANG Ying WANG Fang GAO Tie-jun |
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| Institution: | 1.Shenyang Entry-Exit Inspection and Quarantine Bureau of P.R.C,Shenyang 110016,China;2.The Animal Health Supervision Yi Town in Jinzhou,Jinzhou,Liaoning 121100,China) |
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| Abstract: | By using chicken tissue as test material,selecting the mitochondrial DNA（mtDNA） 12S rRNA gene as marker gene,we designed capture probes,amplified a fragment of 230 bp.No cross-reaction was detected with other 13 animal mitochondrial DNA.The PCR-DHPLC had a detection limit of 2.04 mg/L genomic DNA for chicken tissue.22 feedstuff samples detected 8 chicken tissue positive.These results indicated that the PCR-DHPLC assay could be used for specific rapid detection of chicken tissue in feedstuff. |
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| Keywords: | feedstuff chicken tissue PCR-DHPLC |
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