猪源副黏病毒HX01株分离鉴定、全基因测序与遗传变异分析 |
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引用本文: | 陈胜利,刘辉,唐文雅,郝华芳,张渭东,王兴龙,杜恩岐,张淑霞,党如意,杨增岐.猪源副黏病毒HX01株分离鉴定、全基因测序与遗传变异分析[J].兽医大学学报,2012(10):1418-1422,1428. |
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作者姓名: | 陈胜利 刘辉 唐文雅 郝华芳 张渭东 王兴龙 杜恩岐 张淑霞 党如意 杨增岐 |
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作者单位: | 西北农林科技大学动物医学院,陕西杨凌712100 |
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基金项目: | “十一五”国家科技支撑计划课题(2007-2010) |
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摘 要: | 从陕西省户县某猪场患流感样症状病死仔猪肺脏分离得到1株副黏病毒,命名为猪源副黏病毒(PPMV)HX01株,对分离毒株鉴定后进行部分生物学特性研究和全基因测序分析。参考PPMV JL-1株基因组序列设计10对引物,对PPMV HX01株分段扩增并测序,将测序成功的各序列依次拼接得到全基因序列并进行序列比对。该病毒MDT为91.2h,ICPI和IVPI为0,EID50为10-10.25/mL,表明该毒株属于新城疫弱毒株。序列分析结果表明,PPMV HX01株(全基因序列GenBank登录号:JF795531)与NDV参考毒株全基因核苷酸同源性83.3%~99.8%,该病毒与基因Ⅱ型我国传统弱毒疫苗株La Sota全基因水平和各个基因编码开放阅读框的同源性均在99.5%以上,而与基因Ⅸ型国家标准强毒株F48E9和目前流行的基因Ⅶ型毒株亲缘关系较远。
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关 键 词: | 猪源副黏病毒 新城疫病毒 分离鉴定 全基因组 |
Isolation,identification and genomic analysis of porcine paramyxovirus strain HX01 |
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Authors: | CHEN Sheng-li LIU Hui TANG Wen-ya HAO Hua-fang ZHANG Wei-dong WANG Xing-long DU En-qi ZHANG Shu-xia DANG Ru-yi YANG Zeng-qi |
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Institution: | (College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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Abstract: | A porcine paramyxovirus(PPMV) strain HX01 was isolated from pigs with influenza-like illness in Shaanxi province,China.Biological characteristics and complete genome were obtained.According to PPMV strain JL-1,ten pairs of primers were designed,genome of PPMV strain HX01 was amplified,sequenced and aligned with other NDV strains.The MDT,ICPI,IVPI and EID50 of the strain was 91.2 h,0,0 and 10-10.25/mL,respectively.The complete geonone nucleotide sequence of the strain was logged in the GenBank(GenBank accession:JF795531).Sequence analysis results indicated that the homology of complete genome nucleotide sequence of the strain with NDV reference strains was 83.3%-99.8%,genetically highly close to avirulent strain La Sota(genotypeⅡ),but distant from F48E9 which belongs to genotype Ⅸ and epidemic NDV virus which belongs to genotype Ⅶ. |
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Keywords: | porcine paramyxovirus Newcastle disease virus isolation and identification complete genome |
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