| 猪圆环病毒2型河南株全基因组的克隆与序列分析 |
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| 引用本文： | 王亚丹,卢权威,陈红英,崔保安,王淑娟,朱前磊,王子馨,钞安军.猪圆环病毒2型河南株全基因组的克隆与序列分析[J].兽医大学学报,2012(10):1429-1434. |
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| 作者姓名： | 王亚丹 卢权威 陈红英 崔保安 王淑娟 朱前磊 王子馨 钞安军 |
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| 作者单位： | 河南农业大学牧医工程学院,河南郑州450002 |
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| 基金项目： | 河南省杰出人才创新基金资助项目（0621002100） |
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| 摘 要： | 利用PCR方法,对河南郑州、南阳、焦作等地采集的疑似猪圆环病毒2型（PCV2）感染的病料进行检测。PCR检测为阳性的病料经处理后,接种无PCV污染的PK-15细胞中盲传6代,克隆11株PCV2全基因组并进行序列分析。结果表明,11株PCV2中有10株基因组全长为1 767bp,基因组分型为PCV2b,1株为1 768bp,说明PCV2b是河南省断奶后多系统衰竭综合征（PMWS）发生的主要因素;11个毒株的同源性位于94.9%～100%,与其他毒株的同源性为94.7%～100%;10株基因组为1 767bp的毒株处于一大分支上,遗传进化比较稳定;本试验为PCV2在河南地区的分子流行病学、遗传变异及防治奠定了基础。
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| 关 键 词： | 猪圆环病毒2型 全基因序列 克隆 序列分析 |
Cloning and sequence analysis of the complete genome of porcine circovirus type 2 from Henan province |
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| Authors: | WANG Ya-dan LU Quan-wei CHEN Hong-ying CUI Bao-an WANG Shu-juan ZHU Qian-lei WANG Zi-xin CHAO An-jun |
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| Institution: | (College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | Samples suspiciously infected with porcine circovirus type 2（PCV2） were collected from several regions in Henan and detected by PCR.PCV2-positive samples were inoculated into non-PCV contaminated PK-15 cells and passaged six times.The complete genomes of 11 PCV2 strains were cloned,sequenced and analyzed.The results showed that the genomes length of 10 strains was 1 767 bp and of 1 strain was 1 768 bp,indicating PCV2b was the main causative agent of PMWS in Henan.The homologies among the 11 strains were 94.9%-100%,and they shared 94.7%-100% homologies with other PCV2 strains.Phylogenetic tree showed the 10 PCV2b strains were closely related.This work may provide some basis for studying the molecular epidemiology,variation and prevention of PCV2 in Henan. |
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| Keywords: | PCV2 complete genome cloning sequence analysis |
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