| 利用mRNA差异显示技术克隆分析捻转血矛线虫滞育相关基因 |
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| 引用本文： | 郑柏玲,张红丽,周前进,陈学秋,杜爱芳.利用mRNA差异显示技术克隆分析捻转血矛线虫滞育相关基因[J].兽医大学学报,2012(5):675-681. |
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| 作者姓名： | 郑柏玲 张红丽 周前进 陈学秋 杜爱芳 |
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| 作者单位： | 浙江大学动物科学学院动物预防医学研究所农业部动物疫病病原学与免疫控制重点开放实验室,浙江杭州310058 |
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| 基金项目： | 国家自然科学基金资助项目（30972174 30771618）; 博士点基金资助项目（20100101110102） |
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| 摘 要： | 以培养获得的捻转血矛线虫滞育期虫体及同期正常发育的虫体为研究材料,采用设计的锚定引物和随机引物,通过mRNA差异显示PCR技术对滞育期幼虫的差异表达基因进行了筛选。结果获得了74个滞育期差异表达的基因EST。生物信息学分析表明：29个差异序列与已知的捻转血矛线虫基因组序列具有同源性;14个差异序列与已知线虫的EST具有同源性。同源基因中的rps-30、T24F1.2等已被证明参与了秀丽隐杆线虫的滞育形成。差异序列的克隆为捻转血矛线虫滞育相关基因及滞育形成机制的研究奠定了基础。
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| 关 键 词： | 捻转血矛线虫 滞育 mRNA差异显示 序列分析 |
Screening and identification of diapause-related genes from Haemonchus contortus by mRNA differential display technique |
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| Authors: | ZHENG Bai-ling ZHANG Hong-li# ZHOU Qian-jin CHEN Xue-qiu DU Ai-fang |
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| Institution: | (Key Laboratory of Animal Epidemic Etiology ＆ Immunological Prevention of Ministry of Agriculture,Institute of Preventive Veterinary Medicine,College of Animal Sciences,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | Different genes expressed in diapause of Haemonchus contortus larvae and the normal larvae during the same period were compared and screened by DDRT-PCR with 3′ anchor primers and arbitrary primers.Finally 74 different expression ESTs were obtained,bioinformatics analysis showed that 29 ESTs have homology compared with the known Haemonchus contortus genome,14 ESTs have the homology compared with the known nematode ESTs.Among them,rps-30,T24F1.2 etc have been proved participated in the arrest development of Caenorhabditis elegans.This study has made a foundation for the future research of the related arrested genes and mechanism of diapause in Haemonchus contortus. |
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| Keywords: | Haemonchus contortus diapause（arrested development） mRNA differential display sequence analysis |
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