| 西门塔尔牛CS基因多态性与肉质性状的相关性分析 |
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| 引用本文： | 田静,王晶姝,芦春艳,张路培,杨润军,李俊雅,赵志辉.西门塔尔牛CS基因多态性与肉质性状的相关性分析[J].兽医大学学报,2012(10):1569-1571. |
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| 作者姓名： | 田静 王晶姝 芦春艳 张路培 杨润军 李俊雅 赵志辉 |
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| 作者单位： | [1]吉林大学畜牧兽医学院,吉林长春130062 [2]长春高新技术开发区奋进乡畜禽防疫服务中心,吉林长春130052 [3]中国农业科学院畜牧兽医研究所,北京100193 |
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| 基金项目： | 国家“863”计划资助项目（2008AA101010）; 国家科技支撑计划资助项目（2007BAD55B01） |
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| 摘 要： | 以柠檬酸合成酶基因（citrate synthetase,CS）为候选基因,分析了该基因外显子3的遗传多态性与西门塔尔牛肉质性状的相关关系。选用95头西门塔尔牛为试验材料,采用PCR-RFLP的方法对该基因的外显子多态性进行检测,并利用SPSS软件对该外显子多态性与肉质性状的相关性进行分析。结果表明：CS基因第三外显子第206bp处存在G/A突变,该基因多态性与脂肪颜色呈显著相关,AA型显著高于BB型。对其他性状的影响差异不显著。通过研究CS基因外显子的多态性,为西门塔尔牛利用该基因的多态性进行分子育种提供了试验依据。
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| 关 键 词： | 西门塔尔牛 CS基因 多态性 肉质性状 |
Association between the polymorphism of CS and meat quality traits in Simmental cows |
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| Authors: | TIAN Jing WANG Jing-shu LU Chun-yan ZHANG Lu-pei YANG Run-jun LI Jun-ya ZHAO Zhi-hui |
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| Institution: | 1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Fenjin Town Animal Disease Prevention Center of Changchun,Changchun 130052,China;3.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100193,China) |
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| Abstract: | This experiment was conducted to study an association between the genetic polymorphism of CS exon 3 and meat quality traits in Simmental cows.The PCR-RFLP method was applied to study the genetic polymorphism of CS exon 3 in 95 Simmental cows.And then the genetic correlations between genotypes and performances was obtained by SPSS.The results showed that there were 3 genotypes in Aor13HI,AA,AB and BB.The results indicated that the genetic polymorphisms significantly correlated with fat color,AA genotype was higher than BB genotype（P0.05）.These results indicated that AA is a predominant genotype in Simmental cows. |
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| Keywords: | Simmental cows CS gene polymorphism meat quality |
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