| 滴鼻途径感染猪血凝性脑脊髓炎病毒小鼠的免疫动态变化 |
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| 引用本文： | 何叶,周平,苏高莉,孙秀萍,刘立卓,潘伟,兰云刚,高丰,贺文琦,靳朝.滴鼻途径感染猪血凝性脑脊髓炎病毒小鼠的免疫动态变化[J].兽医大学学报,2012(7):951-954,979. |
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| 作者姓名： | 何叶 周平 苏高莉 孙秀萍 刘立卓 潘伟 兰云刚 高丰 贺文琦 靳朝 |
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| 作者单位： | 吉林大学畜牧兽医学院,吉林长春130062 |
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| 基金项目： | 国家自然科学基金资助项目（31172291） |
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| 摘 要： | 应用石蜡切片、ELISA等方法，通过对小鼠脾脏病理组织学观察以及脾脏指数、IL-2、IFN-γ和TNF-α等重要细胞因子的检测，研究猪血凝性脑脊髓炎病毒（Hemagglutinatingencephalomyelitisvirus，HEV）感染不同周龄（4、6周龄）BALB／c小鼠后的免疫动态变化特点。结果显示，无论是4周龄还是6周龄小鼠，在被HEV感染后的1～3d，脾小体生发中心增大并在红髓有多量浆细胞出现，第4天脾小体开始缩小，其周边和中央动脉周围有多量淋巴细胞聚集，到第5天脾小体生发中心消失并在其周边和中央动脉周围淋巴细胞增多。另外，脾脏指数和血清中的TNF-α、IFN-γ、IL-4浓度均呈现先升高后降低的趋势，IL-2含量变化不明显，而IL-10含量较少几乎检测不到。结果表明，HEV感染初期，小鼠对侵入的病毒做出一定的免疫应答，但是随着病毒复制，病毒量的增大，小鼠的免疫反应受到抑制。同时不同周龄小鼠的被检指标降低或升高程度及持续时间不同，表明HEV的免疫、发病与小鼠感染年龄之间存在一定相关性。
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| 关 键 词： | HEV 小鼠 免疫动态 |
The change of immunity kinesis of mice inoculated with porcine hemagglutinating encephalomyelitis virus by the intranasal |
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| Authors: | HE Ye ZHOU Ping SU Gao-li SUN Xiu-ping LIU Li-zhuo PAN Wei LAN Yun-gang GAO Feng HE Wen-qi JIN Zhao |
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| Institution: | (College of Animal Science and Veterinary Medicine , J ilin Universi ty , Changchun 130062, China) |
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| Abstract: | To analyze the immune occurrence and the impact of the immunesystem of mice inoculated with hemagglutinating encephalomyelitis virus, technology of paraffin sections and ELISA was used to search the pathological chan- ges of spleen, spleen index and the dynamic changes of inductive activity of interleukin-2 （IL-2）, interferon-γ（IFN-γ） and tumor necrosis faetor-α（TNF-α） in serum of 4 weeks or 6-week-old BALB/c mice infected with HEV. The results showed that：4 weeks or 6-week-old mice at 1 to 3 days post-infection,germinal center of splenic corpuscle in- creased and large amounts of plasma cells appeared at the red pulp. At the forth day post-infection, the splenic corpuscle began to shrink and the splenic germinal centers disappeared and lymphocyte increased around the central ar- tery at the fifth day. The spleen index and IFN-γ,TNF-α, IL-4 in serum of the BALB/c mice inoculated with HEV were increased first and then decreased compared with those of control,however the changes of IL-2 and IL-10 were not obvious. The results indicated that above-mentioned index played a very important regulation role in immune pathogenesis of HEV. |
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| Keywords: | HEV mice immunologic dynamics |
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