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| 里氏木霉40359原生质体制备条件研究 | |
| 引用本文： | 张晓烜,李景富,王傲雪.里氏木霉40359原生质体制备条件研究[J].东北农业大学学报,2011,42(8):108-112. |
| 作者姓名： | 张晓烜 李景富 王傲雪 |
| 作者单位： | 1. 东北农业大学成栋学院,哈尔滨,150030 2. 东北农业大学园艺学院,哈尔滨,150030 3. 东北农业大学生命科学学院,哈尔滨,150030 |
| 基金项目： | 哈尔滨市科委留学回国人员项目 |
| 摘 要： | 对影响里氏木霉(Trichoderma reesei )40359原生质体制备和再生的条件:包括茵龄、水解酶液的种类及浓度、酶解温度、酶解时间、再生培养基的稳渗剂进行了研究.结果表明:当茵龄为12 h,采用2%纤维素酶和2%蜗牛酶混合液,酶解温度30℃,酶解时间2h,用0.6 mol·L-1蔗糖作再生培养基的稳渗剂,原... |
| 关 键 词： | 里氏木霉 原生质体 原生质体形成与再生 |
Protoplast preparation in Trichoderma reesei 40359 |
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| ZHANG Xiaoxuan,LI Jingfu,WANG Aoxue.Protoplast preparation in Trichoderma reesei 40359[J].Journal of Northeast Agricultural University,2011,42(8):108-112. | |
| Authors: | ZHANG Xiaoxuan LI Jingfu WANG Aoxue |
| Institution: | 1.College of Chengdong,Northeast Agricultural University,Harbin 150030,China;2.College of Horticulture,Northeast Agricultural University,Harbin 150030,China;3.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China) |
| Abstract: | The protoplast preparation and regeneration conditions in Trichoderma reesei 40359 were studied.The results showed that the optimal condition for protoplast preparation in Trichoderma reesei 40359 was culture mycelia for 12 h,then enzymelysed for 2 h at 30 ℃ by the mixture solution of snail digestase(2%) and cellulase(2%).The protoplasts formation number was 6.14×105·mL-1 and the regeneration rate was 10.6% under the optimal condition when 0.6 mol·L-1 surose used as the osmotic stabilizer. |
| Keywords: | Trichoderma reesei protoplasts preparation and regeneration of protoplasts |
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