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胡柚上柑橘衰退病毒的分子检测
引用本文:武晓云,刘琦,郭玉双,武小霞.胡柚上柑橘衰退病毒的分子检测[J].东北农业大学学报,2011,42(7):130-134.
作者姓名:武晓云  刘琦  郭玉双  武小霞
作者单位:1. 浙江农林大学农业与食品科学学院,浙江临安,311300
2. 黑龙江省农业科学院园艺分院,哈尔滨,150086
3. 贵州省烟草科学研究所,贵阳,550081
4. 东北农业大学农学院,大豆生物学教育部重点实验室,哈尔滨150030
基金项目:国家自然科学基金,浙江农林大学人才启动项目,黑龙江省教育厅项目,哈尔滨市技创新人才研究专项资金项目,东北农业大学人才启动基金
摘    要:胡柚是我国东部地区重要的柑桔栽培品种.近年来,一种以叶片黄化为主要症状的新病害在胡柚种植区暴发流行.通过血清学和分子生物学检测,研究首次从黄化的胡柚叶片中检测到柑橘衰退病毒.系统进化分析表明,分离的CTV CS-7分离物与P09.18、NZRB-TH30等分离物具有较近的进化关系,而与T30等强毒株系有较大的遗传距离,...

关 键 词:胡柚  黄斑病  柑橘衰退病毒  系统进化分析

Detection of Citrus tristeza virus in Huyou(Citrus changshanensis)
wu Xiaoyun,LIU Qi,GUO Yushuang,WU Xiaoxia.Detection of Citrus tristeza virus in Huyou(Citrus changshanensis)[J].Journal of Northeast Agricultural University,2011,42(7):130-134.
Authors:wu Xiaoyun  LIU Qi  GUO Yushuang  WU Xiaoxia
Institution:1.College of Agricultural and Food Science,Zhejiang Agricultural and Forestry University,Lin’an Zhejiang 311300,China;2.Horticulture Sub-branch,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;3.Guizhou Tobacco Research Institute,Guiyang 550081,China;4.College of Agriculture,Northeast Agricultural University,Key Laboratory of Soybean Biology,Ministry of Education,Harbin 150030,China)
Abstract:Huyou(Citrus changshanensis) is an important citrus species in east of China.Recently,a new disease characterized by leaf yellowing broke out in many Huyou planting areas.By using serological and molecular biological methods,Citrus tristeza virus(CTV) was firstly detected from Huyou leaves showing yellow symptoms.Phylogenetic analysis indicated that,comparing to the severe symptom isolates of CTV(such as T30),CS-7 had closer relationship with mild CTV isolates,including P09.18 and NZRB-TH30.The results indicated that CS-7 might represent a new strain of CTV which might be responding for the yellowing symptom on Huyou.
Keywords:Huyou  yellow leaf disease  Citrus tristeza virus  phylogenetic analysis
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