| 番茄大花萼突变体MC基因的AFLP分子标记及种质资源筛选 |
| |
| 引用本文： | 李晓蕾,李景富,许向阳.番茄大花萼突变体MC基因的AFLP分子标记及种质资源筛选[J].东北农业大学学报,2011,42(7):62-66. |
| |
| 作者姓名： | 李晓蕾 李景富 许向阳 |
| |
| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
| |
| 基金项目： | 国家863计划，现代农业产业技术体系建设专项资金，黑龙江省普通高校新世纪人才培养计划 |
| |
| 摘 要： | 采用AFLP分子标记方法,在DNA水平上研究番茄大花萼基因.以番茄大花萼突变体品种08085和正常番茄品种08086为亲本材料,分析鉴定了08086×08085以及其有性杂交F1、F2代分离群体的田间表现.结果表明,番茄花萼突变体MC基因所控制的性状是单显性遗传性状.研究用488对引物筛选出3个与番茄MC基因紧密连锁的...
|
| 关 键 词： | 番茄 MC基因 分子标记 种质资源 筛选 |
AFLP molecular markers of tomato MC gene and screening of germplasmresources |
| |
| LI Xiaolei,LI Jingfu,XU Xiangyang.AFLP molecular markers of tomato MC gene and screening of germplasmresources[J].Journal of Northeast Agricultural University,2011,42(7):62-66. |
| |
| Authors: | LI Xiaolei LI Jingfu XU Xiangyang |
| |
| Institution: | (College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
| |
| Abstract: | Based on AFLP marking method tomato macro calyx gene was studied.Tomato cultivars 08086 and 08085 were used as parents to analyze field performance of F1 and segregating population F2.The results showed that this trait was controlled by single gene.Two parents’ materials and F2 population were analyzed with 488 pairs of primers by AFLP method.Three markers(E32M36-D,E65M63-D and E47M75-A)were obtained and they closely linked to MC gene,their distance was 7.2,5.1 and 12.3 cM,respectively.Total 48 germplasm resources were detected by using AFLP marker E65M63-D combining with identification in the fields,and the results showed high degree of fitness and provided the basis for molecular breeding and gene cloning. |
| |
| Keywords: | tomato MC gene molecular marker germplasm resource screening |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
