Analysis of Accumulation Patterns of <Emphasis Type="Italic">Barley yellow dwarf virus-PAV</Emphasis> (BYDV-PAV) in Two Resistant Wheat Lines |
| |
| Authors: | Florian?Chain Gérard?Riault Maxime?Trottet Email author" target="_blank">Emmanuel?JacquotEmail author |
| |
| Institution: | (1) Biologie des Organismes et des Populations appliquée à la Protection des Plantes [UMR BiO3P], UMR INRA/Agrocampus-Rennes, BP 35327, Domaine de la Motte, 35653 Le Rheu Cedex, France;(2) Amélioration des Plantes et Biotechnologies Végétales [APBV], UMR INRA/Agrocampus-Rennes, BP 35327, Domaine de la Motte, 35653 Le Rheu Cedex, France |
| |
| Abstract: | Barley yellow dwarf (BYD) is one of the main viral diseases of small-grain cereals. This disease, reported on numerous plant
species of the Poaceae family, is caused by a complex of eight viral species including the species Barley Yellow Dwarf Virus-PAV (BYDV-PAV), frequently found in western Europe. Resistance sources against BYDV-PAV are scarce and only identified in perennial
Triticineae. Some BYDV-resistant wheat lines have been obtained by introgressing these resistances into bread wheat germplasms. Genetic
and biological characterization of the resulting lines has been undertaken. However, little information on the resistant behaviour
of these lines during the early stages of the infection process is available. To evaluate the resistance of two genetically
distinct resistant lines (Zhong ZH and TC14), 1740 young plantlets, belonging to susceptible reference hosts (barley cv. Express and wheat cv. Sunstar), Zhong ZH or TC14 wheat lines, were inoculated in controlled conditions with French BYDV-PAV isolates. The infection process was monitored
during the first 21 days after inoculation (DAI) using a semi-quantitative ELISA. A standardized protocol including five successive
samplings of leaves from all inoculated plants and the collection of plant roots at the end of the monitored period was carried
out. This protocol enabled an assessment of the infection percentage and the evolution of the viral load in plants from the
7th DAI to the 21st DAI. Statistical analyses of the BYDV infection kinetics using raw ELISA data, a model of the time-dependent
variation of the percentage of infected plants and the area under concentration progress curves (AUCPC) demonstrated that
Zhong ZH and TC14 lines (1) reduce the development rate of the BYD disease during the first days of infection, (2) decrease the infection efficiency
of BYDV-PAV isolates, in the leaves, from 98.7% for susceptible plant genotypes to 81.9% and 71.7% for Zhong ZH and TC14, respectively, (3) reduce the virus load in the leaves of infected plants and (4) are not spared from BYDV infection, as
95.1% of Zhong ZH and 90.2% of TC14 inoculated plants accumulated viral particles in roots and/or in leaves at 21 DAI. These results confirm the BYDV-partial
resistant behaviour of both Zhong ZH and TC14 lines. The development rate of the disease was the single parameter that allowed the distinction between the two resistant
sources present in the tested lines. |
| |
| Keywords: | AUCPC Luteovirus Poaceae semi-quantitative ELISA TC14 viral exposure Zhong ZH |
| 本文献已被 SpringerLink 等数据库收录！ |
|
