| 大豆组织培养再生植株研究 |
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| 引用本文： | 袁鹰,刘德璞,郑培和,徐文静,李海龙.大豆组织培养再生植株研究[J].大豆科学,2001,20(1):9-13. |
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| 作者姓名： | 袁鹰 刘德璞 郑培和 徐文静 李海龙 |
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| 作者单位： | 1. 吉林省农业科学院生物重点开放实验室,
2. 吉林省农业机械化学校, |
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| 摘 要： | 不同基因型的大豆子叶节在添加适宜BA和IBA的1/2MS培养基上经过二周的预培养和二周芽诱导培养，可获得高频率的丛生芽，及时将丛生芽从高浓度的BA转至低浓度BA培养时芽可长出植株，再转至添加NAA的生根培养基上形成完整的植株。经过40-60天的培养，每个外植体可得到20-30个芽。芽增殖数和植株再生率因基因型、激素、切割位点、诱导时间等不同而有明显差异。初步确立了丛生芽发生和植株再生的适宜条件和程序，建起了以子叶节为外植体适合用于农杆菌介导和基因枪方法进行遗传转化的高频再生体系。
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| 关 键 词： | 大豆 子叶节 丛生芽 植株再生 组织培养 |
| 文章编号： | 1000-9841(2001)01-0009-05 |
STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE |
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| Yuan Ying,Liu Depu,Zheng Peihe,Xu Wenjing,Li Hailong.STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE[J].Soybean Science,2001,20(1):9-13. |
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| Authors: | Yuan Ying Liu Depu Zheng Peihe Xu Wenjing Li Hailong |
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| Institution: | Yuan Ying 1 Liu Depu 1 Zheng Peihe 1 Xu Wenjing 1 Li Hailong 2 |
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| Abstract: | High frequency multiple shoot-bud formation from different genotype of soybean cotyledon node for the two-week pre-culture and two-week shoot-inducing culture on a 1/2MS-medium supplemented with BA and IBA was obtained. Plant can be obtained from multiple shoot-bud when transferred to low concentration of BA, and afterwards to fresh rooting medium supplement NAA. 20-30 buds were obtained from per explant for 40-60 days culture. It was found that the plant regeneration frequency varies obviously from genotypes, hormones , different excision cite and inducing time .Suitable condition and procedure were established for the clump buds formation and plant regeneration, and this high frequency regeneration system can be applied to the soybean genetic transformation by agora-bacterium and particle bombardment. |
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| Keywords: | Soybean Cotyledon Multiple shoot Plant regeneration |
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