| 秋水仙素对黑麦有丝分裂及多倍体诱导的影响 |
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| 引用本文： | 陈于和,秦素平,林小虎,董超华,东方阳.秋水仙素对黑麦有丝分裂及多倍体诱导的影响[J].核农学报,2006,20(4):321-323. |
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| 作者姓名： | 陈于和 秦素平 林小虎 董超华 东方阳 |
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| 作者单位： | 河北科技师范学院生命科学系,河北,秦皇岛,066600 |
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| 摘 要： | 本文研究了秋水仙素在不同浓度(0.01%、0.05%、0.10%、0.15%、0.20%)和不同处理时间(12、24、36、48、60和72h)对黑麦根尖细胞染色体、中期分裂指数、细胞加倍指数的影响。结果表明,秋水仙素处理使黑麦根尖细胞内染色体数目发生了变化,观察到的细胞内染色体数有14、28及56条不等;0.15%的秋水仙素处理24h,细胞的中期分裂指数最大,达到0.202%,同浓度秋水仙素处理36h,细胞加倍指数最高,达到0.096%。
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| 关 键 词： | 黑麦 染色体 秋水仙素 中期分裂指数 细胞加倍指数 |
| 文章编号： | 1000-8551(2006)04-321-03 |
| 收稿时间： | 10 18 2005 12:00AM |
| 修稿时间： | 2005年10月18 |
EFFECTS OF COLCHICINE ON MITOSIS AND POLYPLOID-INDUCTION IN RYE |
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| CHEN Yu-he,QIN Su-ping,LIN Xiao-hu,DONG Chao-hua,DONG Fang-Yang.EFFECTS OF COLCHICINE ON MITOSIS AND POLYPLOID-INDUCTION IN RYE[J].Acta Agriculturae Nucleatae Sinica,2006,20(4):321-323. |
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| Authors: | CHEN Yu-he QIN Su-ping LIN Xiao-hu DONG Chao-hua DONG Fang-Yang |
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| Institution: | Department of Life Sciences, Hebei Normal University of Sciences and Technology , Qinhuangdao , Hebei 066600 |
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| Abstract: | The effects of different colchicines concentration(0.01%,0.05%,0.10%,0.15%,0.20%) and treatment duration(12,24,36,48,56,72h) on mitosis index and cell doubling index of rye root tip cell were studied.The results showed that the observed chromosome numbers were 14,28 and 56.The metaphase mitotic index got as high as 0.202% when tip cell was treated 24 hours in 0.15% colchicines.The cell doubling index reached 0.096% as tip cell treated 36 hours in 0.15% colchicines. |
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| Keywords: | rye chromosome colchicine metaphase mitotic index cell doubling index |
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