| 短时间不同剂量UV-B辐射处理对冬小麦幼苗生理指标的影响 |
| |
| 引用本文： | 张文会,王明卓,苗秀莲,张玉霞,李宝娟.短时间不同剂量UV-B辐射处理对冬小麦幼苗生理指标的影响[J].农业科学与技术,2009,10(5):18-21. |
| |
| 作者姓名： | 张文会 王明卓 苗秀莲 张玉霞 李宝娟 |
| |
| 作者单位： | 聊城大学生命科学学院,山东聊城252059 |
| |
| 基金项目： | 国家自然科学基金项目，聊城大学大学生科技创新项目 |
| |
| 摘 要： | 目的]研究短时间不同剂量UV-B辐射对冬小麦幼苗叶片生理指标的影响，为进一步研究植物应答短时间紫外线照射的机理提供参考。方法]以冬小麦为试验材料，采用15和30μW／cm^2。的UV—B照射强度对冬小麦幼苗进行短时间照射处理。测定指标包括：①反射光谱值。用Unispec光谱仪测定反射光谱，并计算所得参数，评价UV—B处理前后叶片叶绿素、花色素苷、类胡萝卜素含量、水分含量以及光合强度的变化，每处理测定10片叶；②可溶性蛋白含量。采用考马斯亮蓝G-250法测定叶片的可溶性蛋白含量，牛血清蛋白作为对照；③荧光参数（Fn／Fm）：将待测叶片暗反应20min后，用OS-30P荧光仪测定部位同叶色，每处理测定5片叶；④相对电导率。采用Martineau等的方法测定；⑤丙二醛（MDA）：采用邹琦的方法测定。测定项目②～⑤均重复5次。结果】UV—B处理导致叶绿素、可溶性蛋白含量以及含水量都降低，且存在剂量效应，30μW／cm。的降幅大于15μW／cm^2；UV—B辐射导致花色素苷和类黄酮含量下降，尤其是处理后2h下降幅度最大，2种剂量的UV—B处理间差异不大。随着处理时间的延长，花色素苷含量呈逐渐上升趋势，其中，30μW／cm。处理的略高于15μW／cm。处理。UV—B辐射处理对类胡萝卜素含量的影响与花青素相同，均表现为先下降后上升的趋势，2种剂量间差异不大；2种剂量的UV—B处理均抑制了PSⅡ的电子传递，尤其是处理2h的抑制作用最大，4、6和8h的抑制有所缓解，且存在剂量效应；2种剂量的UV-B辐射均抑制了叶片的光合速率，随着照射时间的延长抑制效应加大；MDA和相对电导率在照射2h后明显增加，说明高剂量的UV—B处理对膜系统的损伤较大，低剂量的UV—B处理结果不明显。结论]短时间的UV—B辐射处理对冬小麦幼苗叶片的影响具有剂量效应，高剂量比低剂量的作用效果大。且随着处理时间的延长，受害程度有所减轻，该结果与长时间UV—B照射的结果不同。
|
| 关 键 词： | UV-B辐射 冬小麦幼苗 生理指标 |
Influences of Different UV-B Radiation Treatments in Short Time on Some Physiological Characteristics of Winter Wheat Seedlings |
| |
| ZHANG Wen-hui WANG Ming-zhuo,MIAO Xiu-lian,ZHANG Yu-xia,LI Bao-juan.Influences of Different UV-B Radiation Treatments in Short Time on Some Physiological Characteristics of Winter Wheat Seedlings[J].Agricultural Science ＆ Technology,2009,10(5):18-21. |
| |
| Authors: | ZHANG Wen-hui WANG Ming-zhuo MIAO Xiu-lian ZHANG Yu-xia LI Bao-juan |
| |
| Institution: | (School of Life Sciences, Liaocheng University, Liaocheng 252059) |
| |
| Abstract: | Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ultraviolet irradiation in short time. Method] The winter wheat taken as materials were treated with 15 and 30 pW/cm^2 UV-B radiation, then the physiological indexes such as pigment content and photosynthetic rate were determined. Results] The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 pW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UVB treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2 h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious. Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose. With the increase of treatment time, the damage was alleviated; besides, the result was not similar to that of UV-B radiation in long time. |
| |
| Keywords: | UV-B radiation Winter wheat seedling Physiological index |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
