| 用改良的TRIZOL法快速提取仿刺参和中间球海胆的总RNA |
| |
| 引用本文： | 纪丽凤,程德金,刘长伟,常亚青.用改良的TRIZOL法快速提取仿刺参和中间球海胆的总RNA[J].大连水产学院学报,2009(Z1). |
| |
| 作者姓名： | 纪丽凤 程德金 刘长伟 常亚青 |
| |
| 作者单位： | 大连水产学院农业部海洋水产增养殖学重点开放实验室; |
| |
| 基金项目： | 国家863重大项目(2006AA10A411); 辽宁省农业项目(2007203004); 辽宁省创新团队项目(2007t017); 辽宁省科技攻关项目(2006203003) |
| |
| 摘 要： | 用改良的TRIZOL法和普通TRIZOL法分别提取仿刺参Apostichopus japonica体壁、肠、体腔液和中间球海胆Strongylocentrotus intermedius的管足、性腺、齿间肌的总RNA。结果表明:用普通TRIZOL法提取时间长,电泳条带不很清晰,存在降解且各个组织提取的状况不稳定,杂质量多;而用改良TRIZOL法能够快速提取仿刺参、海胆组织的总RNA,电泳后得到的28S rRNA和18S rRNA条带清晰、完整性好、纯度高、得率高,其A/A为2.04~2.14,总RNA浓度为44.96~115.02 ng/μL。
|
| 关 键 词： | 仿刺参 海胆 总RNA提取 |
Rapid extraction of total RNA from sea cucumber Apostichopus japonica and sea urchin by the modified TRIZOL method |
| |
| JI Li-feng,CHENG De-jin,LIU Chang-wei,CHANG Ya-qing.Rapid extraction of total RNA from sea cucumber Apostichopus japonica and sea urchin by the modified TRIZOL method[J].Journal of Dalian Fisheries University,2009(Z1). |
| |
| Authors: | JI Li-feng CHENG De-jin LIU Chang-wei CHANG Ya-qing |
| |
| Institution: | JI Li-feng,CHENG De-jin,LIU Chang-wei,CHANG Ya-qing(Key Laboratory of Mariculture,Agriculture Ministry,People's Republic of China,Dalian Fisheries University,Dalian 116023,China) |
| |
| Abstract: | The total RNA was extracted from body walls,intestines,and colelomic liquid in sea cucumber Apostichopus japonicus with rich in collagen,polysaccharides and pigments and tube feet,gonad,muscles among teeth of the sea urchin Strongylocentrotus intermedius by improved TRIZOL one step method to replace the traditional TRIZOL method with long period,and unclear electrophoresis bands.The modified TRIZOL method was found to extract RNA in the sea cucumber and the sea urchin rapidly,and to have clear electrophores... |
| |
| Keywords: | Apostichopus japonicas sea urchin total RNA extraction |
| 本文献已被 CNKI 等数据库收录！ |
|
