| 液相芯片及其在粮油主要真菌毒素同步检测中的应用 |
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| 引用本文： | 李 鑫,李培武,,张 奇,张 文,张兆威.液相芯片及其在粮油主要真菌毒素同步检测中的应用[J].中国油料作物学报,2012,34(4):449-454. |
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| 作者姓名： | 李 鑫 李培武 张 奇 张 文 张兆威 |
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| 作者单位： | 1.中国农业科学院油料作物研究所，湖北 武汉 430062；
2.农业部油料作物生物学与遗传育种重点实验室，湖北 武汉 430062；
3.农业部生物毒素检测重点实验室，湖北 武汉 430062；
4.农业部油料产品质量安全风险评估实验室（武汉），湖北 武汉 430062；5.农业部油料及制品质量监督检验测试中心，湖北 武汉 430062
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| 基金项目： | 农业部948项目(2010-G1,2011-G5);国家自然科学基金(31171702,31101299);湖北省自然科学基金(2011CDB358);中国农业科学院油料作物研究所所长基金(1610172010003);国家科技支撑计划(2010BAD01B07,2011BAD02D02,2012BAB19B09);现代农业产业技术体系建设专项(CARS-B) |
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| 摘 要： | 液相芯片整合了分子生物学、免疫学、高分子材料、微流控技术、高速数字信号处理、计算机分析等多方面技术，可实现少量样本高通量定性定量检测。本文主要阐述液相芯片定性与定量分析的基本原理、特点及其在分析领域的应用现状，探讨液相芯片在小分子高通量分析中的工作模式及其当前技术难点，分析黄曲霉毒素等粮油主要真菌毒素免疫分析研究进展，指出液相芯片在粮油主要真菌毒素检测中将有广泛的应用前景。
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| 关 键 词： | 液相芯片 粮油 真菌毒素 高通量 同步检测 |
Suspension array and its application in simultaneous detection of major mycotoxins in cereal and oil |
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| LI Xin,LI Pei-wu,ZHANG Qi,ZHANG Wen,ZHANG Zhao-wei.Suspension array and its application in simultaneous detection of major mycotoxins in cereal and oil[J].Chinese Journal of Oil Crop Sciences,2012,34(4):449-454. |
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| Authors: | LI Xin LI Pei-wu ZHANG Qi ZHANG Wen ZHANG Zhao-wei |
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| Institution: | 1(1.Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062,China; 2.Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China; 3.Key Laboratory of Detection for Mycotoxins,Ministry of Agriculture,Wuhan 430062,China; 4.Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture 430062,China; 5.Quality Inspection and Test Center for Oilseeds Products,Ministry of Agriculture,Wuhan 430062,China) |
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| Abstract: | Suspension array integrated several advanced technologies,including immunology,polymer chemistry,microfluidic,high-speed digital signal processing and computer analysis,to realize qualitative and quantitative detection.In this article,principle,character and current situation of the platform were reviewed,especially technical difficulties in high throughput analysis of small molecule.Current immunoassay research progresses of aflatoxin and other mycotoxins in cereal and oil were focused.With sufficient research conditions for suspension array,simultaneous detection of the major mycotoxins in cereal and oil has an extensive application prospect. |
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| Keywords: | Suspension array Cereal and oil Mycotoxin High-throughput Simultaneous detection |
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