| 种坯处理、不同激素浓度在暗培养条件下对多年生黑麦草愈伤组织诱导的影响 |
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| 引用本文： | 徐定炎,何阳鹏.种坯处理、不同激素浓度在暗培养条件下对多年生黑麦草愈伤组织诱导的影响[J].浙江林业科技,2003,23(2):16-19. |
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| 作者姓名： | 徐定炎 何阳鹏 |
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| 作者单位： | 浙江省衢州市衢江区林业局,浙江,衢州,324022 |
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| 摘 要： | 以多年生黑麦草种子为外随体，研究了在暗培养条件下，不同浓度2，4—D、BA及种胚处理对愈伤组织诱导的影响，试验表明：(1)切胚处理对多年生黑麦草愈伤组织诱导起到很大的促进作用；(2)种胚愈伤组织的诱导宜采用MS 2，4—D 5mg/L的培养基；(3)种子愈伤组织的诱导与种子发芽率无关。
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| 关 键 词： | 种坯处理 激素浓度 暗培养 多年生黑麦草 愈伤组织 诱导 |
| 文章编号： | 1001-3776(2003)02-0016-04 |
| 修稿时间： | 2003年2月11日 |
Effect of Embryo Treatment and Different Hormone Concentration under Dark Culture on Callus Induction of Lolium perenne |
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| XU Ding-yan,HEYang-peng.Effect of Embryo Treatment and Different Hormone Concentration under Dark Culture on Callus Induction of Lolium perenne[J].Journal of Zhejiang Forestry Science and Technology,2003,23(2):16-19. |
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| Authors: | XU Ding-yan HEYang-peng |
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| Abstract: | Study was carried out on effect of different concentration acetic acid(2,4-D), 6-benzylamino purine(BA), embryo treatment under dark culture on callus induction of Lolium perenne with its seeds as explants. The result showed that cutting embryo could improve callus induction; culture medium for embryo callus induction was MS+2, 4-D 5mg/L; there had no relation between callus induction with germination of seed. |
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| Keywords: | Lolium perenne callus seed |
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