| 山羊ADD1基因 exon13～18、intron 13～17序列的克隆分析 |
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| 引用本文： | 朱吉,欧阳叙向,杨仕柳,孙建帮.山羊ADD1基因 exon13～18、intron 13～17序列的克隆分析[J].中国草食动物,2008,28(2):8-12. |
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| 作者姓名： | 朱吉 欧阳叙向 杨仕柳 孙建帮 |
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| 作者单位： | 1. 湖南省畜牧兽医研究所,长沙,410131
2. 湖南生物机电职业技术学院 |
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| 基金项目： | 湖南省农业厅项目(03-01-09)，湖南省教育厅项目(05D047) |
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| 摘 要： | 提取湘东黑山羊基因组总DNA,用所设计的引物以聚合酶链式反应扩增山羊ADD1基因,并进行克隆测序。通过对克隆所得片段的测序结果分析,得到了山羊ADD1基因外显子(exon)13~18、内含子(intron)13~17序列,并将序列提交GenBank,获两个序列号:DQ483057、DQ455606;对编码序列(exon 13~18)与牛、人同区域进行Blast对比,同源性分别达到了97.43%和86.12%。聚类分析结果表明,在6个物种中,牛与山羊ADD1基因的同源性最高,其它几个物种间同源系数相差不大,在83%至89%之间。
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| 关 键 词： | 山羊 ADD1 序列分析 克隆 |
| 文章编号： | 1007-9726(2008)02-0008-05 |
| 修稿时间： | 2007年8月21日 |
Cloning and Sequence Analysis of Goat ADD1 Gene exon 13-18 and intron 13-17 |
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| Zhu Ji,Ouyang Xu-xiang,Yang Shi-liu,et al.Cloning and Sequence Analysis of Goat ADD1 Gene exon 13-18 and intron 13-17[J].China Herbivores,2008,28(2):8-12. |
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| Authors: | Zhu Ji Ouyang Xu-xiang Yang Shi-liu |
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| Institution: | Zhu Ji1,Ouyang Xu-xiang2,Yang Shi-liu1,et al |
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| Abstract: | Extracting total DNAs from Xiangdong Black Goat,we amplified Hircine ADD1 Gene by polymerase chain reaction using one primers,then colon and sequence them.That Combining and analysing of Sequencing results of one PCR-derived fragments showed we obtained exon13~18 and intron 13~17 of Hircine ADD1 Gene,Sending the sequences to GenBank and we received two accession number:DQ483057,DQ455606;the homologous percentage of coding regions(exon13~18) were 97.43%,86.12% separately when blasting it with the bovine and human being,further more,the results of blast the sequences with other five species,the sequence homology of goat ADD1 gene and cow was the highest ,and the of homology index among the goat ADD1 gene and the other species were between 83% and 89%,the differences was little. |
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| Keywords: | goat ADD1 sequence analysis cloning |
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