| 甘蓝型油菜子叶原生质体培养再生植株 |
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| 引用本文： | 孟征,李世君,李德葆.甘蓝型油菜子叶原生质体培养再生植株[J].浙江大学学报(农业与生命科学版),1993(4). |
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| 作者姓名： | 孟征 李世君 李德葆 |
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| 作者单位： | 浙江农业大学生物技术研究所，浙江农业大学生物技术研究所，浙江农业大学生物技术研究所 杭州 310029，杭州 310029，杭州 310029 |
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| 基金项目： | 浙江省科委“八五”重点课题 |
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| 摘 要： | 从早熟甘蓝型油菜品种601的子叶中游离出原生质体,采用液体浅层培养,获得持续分裂的细胞。培养基中的2,4-D浓度对刺激细胞分裂至关重要。形成的小愈伤组织必须经继代培养(MS培养基补加2,4-D 0.2mg/L,KT 2mg/L)后,再转至MS分化培养基(补加NAA0.01mg/L,KT 3mg/L)上,才能分化出芽。分化的芽在MS生根培养基(补加IBA0.5mg/L)上,可以形成完整植株。
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| 关 键 词： | 甘蓝型油菜 原生质体 植株再生 |
Regenerated plant from cotyledon protoplasts of Rrassica napus. |
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| Meng Zeng,Li Shijun, Li Debao.Regenerated plant from cotyledon protoplasts of Rrassica napus.[J].Journal of Zhejiang University(Agriculture & Life Sciences),1993(4). |
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| Authors: | Meng Zeng Li Shijun Li Debao |
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| Abstract: | Protoplasts of Brassica napus were isolated from cotyledon explant. Cell division took place and calli were obtained on thin-layer liquid medium. Sustained cell division was attributed to the presence of 2. 4-D in the medium. Young shoot regeneration occurred from the cotyledon-derived calli,which had been proliferated on the MS medium supplied with hormones of 2. 4-D 0. 2 mg/L and KT 2 mg/L.then being transferred onto a modified MS medium supplied with NAA 0. 01 mg/L.KT 3 mg/L. After the shoots were transferred onto the rooting medium supplied with IBA 0, 5 mg/L .intact plants were eventually produced. |
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| Keywords: | Brassica napus L protoplasts plant regeneration |
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