Intraspecific differentiation and geographical distribution of Wx alleles for low amylose content in endosperm of foxtail millet, Setaria italica (L.) Beauv. |
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| Authors: | Hiroki Nakayama Muhammad Afzal Kazutoshi Okuno |
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| Institution: | (1) National Institute of Agrobiological Resources, Kannondai 2-1-2, Tsukuba, Ibaraki 305, Japan;(2) National Agricultural Research Centre, Plant Genetic Resources Institute, Islamabad, Pakistan |
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| Abstract: | To clarify the genetic mechanism which controls the variation in amylose content among nonwaxy landraces of foxtail millet,
the inheritance of different starch types in endosperm was examined by I2-KI staining. The level of starch granule bound protein in foxtail millet endosperm was also analyzed using SDS-PAGE. The
segregation for starch types in F2 and F3 seeds determined by I2-KI staining showed that there are three different alleles at the waxy (wx) locus. A major protein bound to starch granules
was detected in nonwaxy endosperm but it was absent in most of the waxy endosperm, suggesting that the protein is the Wx gene
product which is responsible for the synthesis of amylose in endosperm. The level of Wx protein proportionally corresponded
to the amylose content among nonwaxy landraces. This implies that two different Wx alleles regulate quantitative levels of
Wx protein. Landraces with the allele for low amylose content are from Taiwan, the Philippines, Indonesia and Thailand.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | amylose content genetic variation Setariaitalica Wx alleles Wx protein |
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