| 木质素降解菌的筛选及对秸秆的降解研究 |
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| 引用本文： | 梁军锋,张洪生,张克强,张金凤,李野,赵润.木质素降解菌的筛选及对秸秆的降解研究[J].华北农学报,2009,24(5). |
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| 作者姓名： | 梁军锋 张洪生 张克强 张金凤 李野 赵润 |
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| 作者单位： | 农业部环境保护科研监测所,资源再生研究室,天津,300191 |
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| 基金项目： | 农业部农业结构调整重大技术研究专项，中央公益性科研院所基本科研业务费专项 |
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| 摘 要： | 为筛选木质素高效降解菌,对筛选的方法体系进行了研究.结果表明,利用三种定性筛选平板结合定量的木质素磺酸钙降解的方法,能明显提高筛选效率,从16个菌株中筛选到3株对木质素有较强降解能力的菌株Tp1、Tf1与Ls21.为进一步验证所筛菌株的降解效果,选取其中综合性能较好的Tf1菌株(凤尾菇,Pleurotus sajor-caju),对小麦秸秆与玉米秸秆进行固态发酵试验,并以黄孢原毛平革菌(Phanerochaete chrysosporium)BKMF-1767作参照,结果表明,Tf1对小麦秸秆与玉米秸秆木质素的降解率分别为38.4%与47.7%,均高于参照菌株(分别为32.2%和34.6%).
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| 关 键 词： | 秸秆 木质素 筛选 降解 白腐菌 |
Study on the Screening of the Lignin-degrading Fungus and the Degradation of Straws |
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| LIANG Jun-feng,ZHANG Hong-sheng,ZHANG Ke-qiang,ZHANG Jin-feng,LI Ye,ZHAO Run.Study on the Screening of the Lignin-degrading Fungus and the Degradation of Straws[J].Acta Agriculturae Boreali-Sinica,2009,24(5). |
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| Authors: | LIANG Jun-feng ZHANG Hong-sheng ZHANG Ke-qiang ZHANG Jin-feng LI Ye ZHAO Run |
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| Abstract: | To use three kinds of screening plate, and to combine with quantitative degradation test of calcium ligno-sulphonate,three lignin-degradation strains,TP1 ,Tfl and Ls21 were obtained.In order to confirm the degradation effect of the strains, the strain Tf1 ( Pleurotus sajor-caju ) , was selected to do solid substrate fermentation test about the wheat straw and corn straw. And Phanerochaete chrysosporium (BKMF-1767) was made as a reference. The result showed that the degradation rate of Tf1 to the wheat straw and corn was 38 .4% and 47 .7% respectively,and it was higher than the refer?ence strain(32.2% and 34.6% ) .This research improves the screening method system of lignin-degrading fungus.There is an important significance to expand the resource of lignin-degrading fungus and exploit the straw resource. |
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| Keywords: | Straw Lignin Screening Degradation White rot fungi |
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