| 市售草鱼鳃中细菌的分离鉴定及耐药性分析 |
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| 引用本文： | 吕爱军.市售草鱼鳃中细菌的分离鉴定及耐药性分析[J].中国农学通报,2014,30(32):43-47. |
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| 作者姓名： | 吕爱军 |
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| 作者单位： | 江苏师范大学生命科学学院 |
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| 基金项目： | 国家自然科学基金项目“嗜水气单胞菌诱导斑马鱼皮肤免疫应答的分子机制”(30800847)，江苏省“青蓝工程”(2014)。 |
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| 摘 要： | 对徐州市售草鱼细菌带染状况及耐药性分析，结果表明从其鳃中分离到4 株革兰氏阴性短杆菌，暂时编号为ZYF-1、ZYF-2、ZYF-3 和ZYF-4，查阅数值编码鉴定ZYF-1 菌株为志贺（Shigella sp.），ZYF-2、ZYF-3 菌株为大肠埃希菌（Escherichia coli），ZYF-4 菌株为泉居沙雷菌（Serratia fonticola）。药敏试验结果表明，4 株分离菌的耐药率分别为75.8%、72.4%、65.5%和82.8%，均对氨苄西林、阿莫西林、复方新诺明、磺胺异恶唑和甲氧苄啶等11 种抗生素耐药。本研究显示市售草鱼存在志贺菌、大肠埃希菌带染现象，对多种抗生素耐药，这为食源性细菌鉴定及鱼病防治提供参考。
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| 关 键 词： | 产量 产量 |
| 收稿时间： | 3/7/2014 12:00:00 AM |
| 修稿时间： | 3/7/2014 12:00:00 AM |
Identification and Antimicrobial Resistance Analysis of Bacteria Isolated from the Gills of Commercial Grass Carp (Ctenopharyngodon idellus) |
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| Institution: | Hu Xiucai, Lan Yun, Shen Xiaojing, Lv Aijun, Zhu Aihua (1Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou Jiangsu 221116, 2School of Life Sciences, Jiangsu Normal University, Xuzhou Jiangsu 221116) |
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| Abstract: | Investigation was conducted on bacterial contamination and antimicrobial resistance of the strains isolated from the gills of commercial Grass carp（Ctenopharyngodon idellus） in Xuzhou, four isolated strains were gram-negative and short rod-shaped bacteria, and tentatively named ZYF-1, ZYF-2, ZYF-3 and ZYF-4, respectively. According to the values of coding method of four strains, ZYF-1 was identified as Shigella sp.,ZYF-2 and ZYF-3 were identified as Escherichia coli, and ZYF-4 was identified as Serratia fonticola. The antimicrobial susceptibility assay indicated that the resistance rates of ZYF-1, ZYF-2, ZYF-3 and ZYF-4 strains were 75.8%、72.4%、65.5% and 82.8%, respectively. Four strains showed antibiotic resistance to eleven kinds of antibiotics, such as ampicillin, amoxicillin, trimethoprim- sulfamethoxazole, sulfisoxazole,trimethoprim and so on. It is concluded that there are Shigella and Escherichia coli contamination in commercially available Ctenopharyngodon idellus in Xuzhou, and the bacteria showed multi-drug resistances in antimicrobial susceptibility assay. The conclusion will provide scientific references for identification of foodborne bacteria and control of bacterial disease in fish. |
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| Keywords: | Ctenopharyngodon idellus gill isolation and identification antimicrobial susceptibility assay |
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