| V型小麦细胞质雄性不育“三系”及杂交种线粒体DNA的比较研究 |
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| 作者姓名： | liubs@sdau.edu.cn |
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| 作者单位： | 山东农业大学农学院作物生物学重点实验室 山东泰安271018 |
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| 基金项目： | 国家自然科学基金项目“用恢复基因的近等基因系研究细胞质雄性不育性的机理”（30370879）. |
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| 摘 要： | 以V型小麦细胞质雄性不育系及相应的保持系、恢复系以及其杂种F1为材料,用ISSR、SRAP、RAPD分子标记技术对它们的线粒体DNA进行了比较分析。结果如下:(1)153个SRAP引物组合中139个组合扩增出了多态性,9个组合扩增出不育系特有而保持系、恢复系、F1均缺失的片段,3个组合扩增出不育系特异缺失的片段。(2)92个ISSR引物中45个扩增出了多态性,筛选到了6个不育系特有而保持系、恢复系及杂种F1均缺失的片段。(3)288个RAPD引物中281个扩增出了多态性,4个引物扩增出不育系特有的片段,3个引物扩增出不育系特异缺失的片段。在对线粒体DNA进行分析时,SRAP比ISSR多态性好,比RAPD稳定性好。
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| 关 键 词： | 细胞质雄性不育 mtDNA SRAP ISSR RAPD |
Comparative Studies of mtDNAs from the three Lines and their Hybrid of Wheat V- CMS |
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| Authors: | Q |
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| Institution: | Key laboratory of Crop Biology, Agronomy Department, Shandong Agricultural University, Taian Shsndong 271018 |
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| Abstract: | Near isogenic lines of restoring gene to V-type CMS of wheat were bred. The mtDNA of cytoplasmic male sterile line, maintainer line, restorer line and F1 hybrid were compared by using ISSR?SRAP and RAPD techniques. The results are as follows: (1) 153 SRAP primers were screened, 139 of them had amplification polymorphisms, 9 primers had fragments that specific existed in cytoplasmic male sterile line, 3 primers had fragments that didn''t exist in cytoplasmic male sterile line. (2) Ninety-two ISSR primers were screened, 6 primers had fragments that specific existed in cytoplasmic male sterile line. (3) Two hundred and eighty-eight RAPD primers were screened, 281 of them had amplification polymorphism, and 4 primers had fragments that specific existed in cytoplasmic male sterile line, 3 primers had fragments that didn''t exist in cytoplasmic male sterile line. SRAP technique is more polymorphism than ISSR, and is more stable than RAPD when analyzing mtDNA. |
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| Keywords: | Cytoplasmic male sterility Mitochondrial DNA SRAP ISSR RAPD |
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