| 新疆哈密瓜细菌性斑点病种子带菌的分离检测 |
| |
| 引用本文： | 王爱英,李国英,曾义,王钦英.新疆哈密瓜细菌性斑点病种子带菌的分离检测[J].安徽农学通报,2006,12(9):159-160,30. |
| |
| 作者姓名： | 王爱英 李国英 曾义 王钦英 |
| |
| 作者单位： | 1. 石河子大学生物工程学院,新疆石河子,832000
2. 石河子大学农学院,新疆石河子,832000 |
| |
| 基金项目： | 新疆兵团重点项目《甜瓜病害综合治理技术研究与示范》 |
| |
| 摘 要： | 甜瓜果腐病是一种检疫性病害,可随种子远距离传播,给甜瓜生产带来很大的危害。本研究对哈密瓜种子上携带的瓜类果腐病菌和黄瓜角斑病菌进行了育苗症状检测、直接分离和带菌部位测定。结果表明,甜瓜细菌性果腐病和黄瓜细菌性角斑病均可随种子传播,特别是甜瓜细菌性果腐病种子带菌率较高,而且种子内外都携带一定量的病原菌,但种子带菌以种皮带菌为主。将带菌种子于低温下在PBS缓冲液的浸提液中浸提,2-4小时内的浸提效果较好,得到较充分病原菌的富集。
|
| 关 键 词： | 哈密瓜细菌性斑点病 种子检测 浸提 西瓜果腐病菌 黄瓜角斑病菌 |
| 文章编号： | 1007-7731（2006）09-159-02 |
| 收稿时间： | 2006-08-23 |
| 修稿时间： | 2006-08-23 |
Direct isolation and detection of bacterial spot pathogens carrying from Hami melon seeds in Xijiang province |
| |
| Wang Aiying,Li Guoying,Zen Yi,Wang Qinying.Direct isolation and detection of bacterial spot pathogens carrying from Hami melon seeds in Xijiang province[J].Auhui Agricultural Science Bulletin,2006,12(9):159-160,30. |
| |
| Authors: | Wang Aiying Li Guoying Zen Yi Wang Qinying |
| |
| Institution: | 1 Institute of Biology Engineering University in Shi Hezi 2 Agricutural Institute University in ShiHezi |
| |
| Abstract: | The fruit rot of hami melon is a kind of quarantine disease which spreads with seeds in the longer distance. The disease causes severe damages. Hami melons the seeds carrying Pseudomonas syringae pv. Lachrymans and A, avenae subsp. citrull were detected by symptom of seeding,isolating seeds and position carrying pathogenic. The result make clear that the two pathogens spread with seeds. In special, the ratio seeds is higher with A. avenac subsp, citrull. The inner and outer of seeds carry the pathogens and the seed coat is main position . The seeds carrying pathogens soaked in PBS buffer in lower temperature may rich in plenty of pathogens. |
| |
| Keywords: | Hami Melon Bacterial Spot Disease Seeds Detected Soaking Pseudomonas syringae pv Lachrymans A avenae subsp citrull |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
