| Dot-ELISA间接法检测鸡产蛋下降综合症的研究 |
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| 引用本文： | 王文志,颜世敢.Dot-ELISA间接法检测鸡产蛋下降综合症的研究[J].山东农业科学,1998(5):15-18. |
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| 作者姓名： | 王文志 颜世敢 |
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| 摘 要： | 常规方法分离纯化鸡ＩｇＧ，免疫山羊制备羊抗鸡ＩｇＧ二抗，以辣根过氧化物酶（ＨＲＰ）标记制备酶标记物的工作浓度为１∶１０００，选用硝酸纤维素膜（ＮＣ）作固相载体，建立检测ＥＤＳＶ抗原的斑点间接酶联免疫吸附试验诊断方法。检测ＥＤＳＶ鸭胚毒４０份，阳性检出率１００％；人工感染发病鸡的卵巢、输卵管、泄殖腔拭子各５０份，阳性检出率分别为１００％、１００％、９４％。与传染性支气管炎、新城疫、传染性法氏囊病无交叉反应，试验显示该方法简便、特异、快速、结果直观、便于生产应用。
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| 关 键 词： | Dot-ELISA 鸡 产蛋下降综合症 |
Study on Indirect Dot-ELISA to Inspect Egg Drop Syndrome(EDS) |
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| Abstract: | The method indirect Dot-ELISA to inspect EDS antigen has been built up,the working conditions are as followed:(i)using common method to separate and purify chicken IgG;(ii)immuning goat to prepare goat anti-chicken IgG;(iii)labeling goat anti-chicken IgG with Horseradish Peroxides(HRP) to prepare HRP-IgG,with the working density of HRP-IgG 1∶1000;(iv)using NC membrane as solid carrier.By this method,40 EDSV duck embryo allantoic fluid samples have been inspected,all of them are positive.50 samples of common chicken ovary,oviduct and cloaca swab respectively are artifically infected,the positive radials are 100%,100%,94%.There is no cross reaction with Infectious Bronchitis Virus(IBV),Newcastle Disease Virus(NDV) and Infectious Bursal Disease Virus(IBDV).The result show this method is simple,special,rapid,result evident and easy to use in animal production. |
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| Keywords: | Dot-ELISA Egg Drop Syndrome(EDS) |
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