In order to provide suitable parent materials for malting barley, protein content andβandαamylase activity of 13cultivars were analyzed after they were subjected to selective breeding of ecological adaptability and production applicability. The results showed that β-amylase activity varied where it was significantly higher in hu mail 16 than those in others (P<0.05), then was A7-30 which was followed by jin barley2, those in 09-189, zhu3, gang1 and Han 98-58 were statistically lower than above cultivars (P<0.05); grain protein content in Jin barely 2, Lin 3,W-03 and Zhu 9125 were less than 12%; those in 09-189, A7-30, Jing 984 and Yan 95137 were between 12% and 12.5% which were higher than the rest 5 cultivals (P<0.05). Jin barley 2, 09-189 and A7-30 were the suitable parent materials for breeding malting barley since the higher β-amylase activity and than 12.5% grain protein content presented.In order to provide suitable parent materials for malting barley, protein content andβandαamylase activity of 13cultivars were analyzed after they were subjected to selective breeding of ecological adaptability and production applicability. The results showed that β-amylase activity varied where it was significantly higher in hu mail 16 than those in others (P<0.05), then was A7-30 which was followed by jin barley2, those in 09-189, zhu3, gang1 and Han 98-58 were statistically lower than above cultivars (P<0.05); grain protein content in Jin barely 2, Lin 3,W-03 and Zhu 9125 were less than 12%; those in 09-189, A7-30, Jing 984 and Yan 95137 were between 12% and 12.5% which were higher than the rest 5 cultivals (P<0.05). Jin barley 2, 09-189 and A7-30 were the suitable parent materials for breeding malting barley since the higher β-amylase activity and than 12.5% grain protein content presented.