Ornamental plants and thrips populations associated with tomato spotted wilt virus in Greece |
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| Authors: | E K Chatzivassiliou I Livieratos G Jenser N I Katis |
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| Institution: | (1) Plant Pathology Laboratory, Faculty of Agriculture, Aristotle University of Thessaloniki, 540 06 Thessaloniki, Greece;(2) Present address: Dept. of Biology, Imperial College of Science, Technology & Medicine, SW7 2A2 London, UK;(3) Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary |
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| Abstract: | A survey was conducted in order to record the ornamental plants that are hosts of tomato spotted wilt virus (TSWV) and impatiens
necrotic spot virus (INSV) in Greece. Polyclonal antibodies prepared against the N protein of a Greek isolate of TSWV fromGerbera jamesonii (GR-34) were used. Leaf samples were taken from plants showing typical symptoms of tospovirus infection such as chlorotic
and necrotic rings on the leaves and malformation and necrosis of the flowers. The samples were tested by double antibody
sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using polyclonal antibodies to the N proteins of TSWV and INSV (NL-07).
ELIS A-positive samples were mechanically transmitted to plants ofPetunia hybrida, Nicotiana rustica andN. benthamiana to confirm infection. Although none of the samples was found infected with INSV, TSWV presence was recorded in 42 botanical
species that belong to 40 genera in 27 families. Among them the speciesBeloperone guttata, Coleus barbatus, Impatiens petersiana andLilium auratum are reported for the first time as hosts of TSWV, whereasBegonia sp.,Catharanthus roseus Celosia cristata, Dianthus chinensis, Fuchsia hybrida andStephanotis floribunda are found as new hosts of the virus in Greece. Thrips collected from TSWV-infected plants were in most cases identified asFrankliniella occidentalis, except from plants ofDendranthema sp. andDianthus caryophyllus whereThrips tabaci individuals were also identified. Different percentages of transmitters were noticed when the thrips populations collected
from TSWV-infected ornamental hosts were tested for transmission of TSWV. |
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| Keywords: | Ornamental hosts ELISA Frankliniella occidentalis Thrips tabaci Thripidae transmission efficiency |
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