A new male sterile mutant LZ in wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) |
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| Authors: | Kuanji Zhou Shihong Wang Yuqin Feng Wanquan Ji Genxuan Wang |
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| Institution: | (1) Crops Research Institute, Gansu Academy of Agricultural Science, Lanzhou, Gansu, 730070, China;(2) Laboratory of Arid Agroecology, Lanzhou University, Lanzhou, Gansu, 730070, China;(3) Wheat Research Center, Northwest A & F University, Yangling, Shanxi, 712100, China; |
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| Abstract: | Genetic male sterility (GMS) genes in wheat (Triticum aestivum L.) can be used for commercial hybrid seed production. A new wheat GMS mutant, LZ, was successfully used in the 4E-ms system
for producing hybrid wheat, a new approach of producing hybrid seed based on GMS. Our objective was to analyse the genetic
mechanism of male sterility and locate the GMS gene in mutant LZ to a chromosome. We firstly crossed male sterile line 257A
(2n = 42) derived from mutant LZ to Chinese Spring and several other cultivars for determining the self-fertility of the F1 hybrids and the segregation ratios of male-sterile and fertile plants in the F2 and BC1 generations. Secondly, we conducted nullisomic analysis by crossing male sterile plants of line 257A to 21 self-fertile nullisomic
lines as male to test the F1 fertilities and to locate the GMS gene in mutant LZ to a chromosome. Thirdly, we conducted an allelism test with Cornerstone,
which has ms1c located on chromosome 4BS. All F1s were male fertile and the segregation ratio of male-sterile: fertile plants in all BC1 and F2 populations fitted 1:1 and 1:3 ratios, respectively. The male sterility was stably inherited, and was not affected by environmental
factors in two different locations or by the cytoplasm of wheat cultivars in four reciprocal cross combinations. The results
of nullisomic analysis indicated the gene was on chromosome 4B. The allelism test showed that the mutant LZ was allelic to
ms1c. We concluded that the mutant LZ has common wheat cytoplasm and carries a stably inherited monogenic recessive gene named
ms1g. |
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