| Experiment on Cryopreservation of Porcine Oocytes by Using Glass Micropipette |
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| 作者姓名： | 朱良成 芮荣 杨德吉 张德福 刘东 |
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| 作者单位： | 1. 南京农业大学动物医学院,南京,210095
2. 上海市农业科学院畜牧兽医研究所,上海,201106 |
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| 基金项目： | 上海市科技兴农重点攻关项目(沪农科攻字[2003]第14-1号)，国家自然科学基金(No.30270958)资助 |
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| 摘 要： | 用自制的玻璃微管（glass micropipette，GMP）冷冻猪卵母细胞。以FDA染色鉴定冷冻-解冻后卵母细胞存活力，结果表明，用常规细管程序化冷冻和GMP管玻璃化冷冻猪成熟（MⅡ期）卵母细胞时，冻后存活率分别为34.5%和63.3%（P <0.05）；以玻璃化冷冻比较细管和GMP管对猪MⅡ期卵母细胞冷冻效果的影响，两组冻后存活率分别为45.0%和65.9%（P < 0.05）；用常规细管程序化和GMP管玻璃化冷冻猪GV期卵母细胞时，冻后存活率为30.0%对59.7%，有显著性差异（P < 0.05）。研究表明，GMP法为一种有效的猪卵母细胞冷冻保存方法。
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| 关 键 词： | 玻璃微管法 卵母细胞 冷冻保存 猪 |
| 文章编号： | 1006-1304（2006）05-0697-04 |
| 收稿时间： | 2005-12-27 |
| 修稿时间： | 2006-03-07 |
Experiment on Cryopreservation of Porcine Oocytes by Using Glass Micropipette |
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| ZHU Liang-cheng,RUI Rong,YANG De-ji,ZHANG De-fu,LIU Dong.Experiment on Cryopreservation of Porcine Oocytes by Using Glass Micropipette[J].Journal of Agricultural Biotechnology,2006,14(5):697-700. |
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| Authors: | ZHU Liang-cheng RUI Rong YANG De-ji ZHANG De-fu LIU Dong |
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| Institution: | 1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; 2. Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China |
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| Abstract: | The self-made glass micropipette(GMP) was used for cryopreservation of pig oocytes, obtaining good results. The post-thaw survival rate of oocytes was assessed by fluorescein diacetate(FDA) staining. And the results showed that the programmed freezing method and GMP vitrification method respectively resulted in the post-thaw survival rate of 34.5% and 63.3%(P <0.05) in cryopreservation of porcine MⅡstage oocytes. Comparing plastic straw with GMP in vitrification of porcine MⅡ stage oocytes, the survival rates of both groups were 45.0% and 65.9% (P < 0.05), respectively. When GV-stage pig oocytes were cryopreserved by straw programmed freezing method and GMP vitrification method, they yielded survival rates of 30.0% and 59.7% respectively and there was significant difference between them(P < 0.05). The present study shows that GMP method is an efficient freezing method for porcine oocyte cryopreservation. |
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| Keywords: | glass micropipette (GMP) oocytes cryopreservation pig |
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