| 分子标记辅助鉴定小麦抗白粉病品种(系)所含Pm基因 |
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| 引用本文： | 刘金元,陶文静,段霞瑜.分子标记辅助鉴定小麦抗白粉病品种(系)所含Pm基因[J].植物病理学报,2000,30(2):133-139. |
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| 作者姓名： | 刘金元 陶文静 段霞瑜 |
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| 作者单位： | 1. 南京农业大学细胞遗传所, 南京 210095;2. 中国农业科学院植物保护研究所, 北京 100094 |
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| 基金项目： | 国家自然科学基金! (39770 413)，“86 3”项目! (Z- 17- 0 4- 0 1)，农业部攻关项目! (96 - 0 0 2 - 0 2 - 0 30 3) |
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| 摘 要： | 借助与Pm2及Pm4紧密连锁的RFL P标记,对南京农业大学细胞遗传所与江苏省里下河地区农科所合作育成的抗白粉病小麦新品系及其它单位育成或引自国外但所含具体Pm基因尚有异议的抗病品种进行了分析。结果表明,本所与江苏省里下河地区农科所合作育成的抗病新品系及江苏省农科院育成的白粉病抗源材料中所含具体Pm基因与按系谱推导及对白粉病菌抗谱分析的结果相一致。而据单体分析认定为"Pm2x"和"KG"(6A)的白免3号和肯贵阿1号和按侵染型推导为含Pm2+6的"郑州831",在本研究中发现这3个材料所含主效抗病基因均为Pm4,而无Pm2,此结果与分菌系、分小种抗谱鉴定结果相符。另外,RFLP分析还证明了2个引自国外且抗病性已降低的Pm6鉴别寄主Timgalen和CI12632/8Cc所携带Pm6基因已丢失。
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| 关 键 词： | 分子标记技术 普通小麦 白粉病抗性 Pm基因 |
| 修稿时间： | 1999-02-04 |
MOLECULAR MARKER ASSISTED IDENTIFICATION OF Pm GENES INVOLVED IN THE POWDERY MILDEW RESISTANT WHEAT CULTIVARS (LINES) |
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| LIU Jin-yuan ,TAO Wen-jing ,DUAN Xia-yu ,XIANG Qi-jun ,LIU Da-jun ,CHEN Pei-du.MOLECULAR MARKER ASSISTED IDENTIFICATION OF Pm GENES INVOLVED IN THE POWDERY MILDEW RESISTANT WHEAT CULTIVARS (LINES)[J].Acta Phytopathologica Sinica,2000,30(2):133-139. |
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| Authors: | LIU Jin-yuan TAO Wen-jing DUAN Xia-yu XIANG Qi-jun LIU Da-jun CHEN Pei-du |
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| Institution: | 1. Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China;2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | The Pm genes contained in wheat cultivars (lines) developed by Cytogenetics Institute of Nanjing Agricultural University in corporation with Lixiahe Agricultural Research Institute of Jiangsu Province and those from other sources were identified using RFLP markers tightly linked to Pm2 and Pm4a genes. The Pm genes identified in most of the tested materials by molecular markers were in accordance with these by pedigree deduction and analysis of resistance spectrum. But, the statements previously made about "Baimian No.3", "Kenguia No.1" and "Zhengzhou 831" containing "Pm2x", "KG" (6A) and Pm2+6 respectively, were not coincided with the results based on the molecular marker analysis which showed that all above mentioned three cultivars contain Pm4 gene, instead of Pm2 gene. The results were also in agreement with that from resistance spectrum analysis. Two cultivars used as Pm6 differential hosts Timgalen and CI12632/8Cc introduced from IWWPMN, in which the resistance was degraded, were confirmed the Pm6 gene had been lost by RFLP analysis. |
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| Keywords: | molecular marker Triticum aestivum powdery mildew resistance Pm gene |
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