| 籽用大麻性别连锁标记的验证及SCAR标记开发 |
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| 引用本文： | 赵铭森,方书生,陈瑶,康红梅,高金虎,陈思远,杨昕,冯旭平,张立武.籽用大麻性别连锁标记的验证及SCAR标记开发[J].热带作物学报,2019,40(10):2076-2082. |
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| 作者姓名： | 赵铭森 方书生 陈瑶 康红梅 高金虎 陈思远 杨昕 冯旭平 张立武 |
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| 作者单位： | 1. 山西省农业科学院经济作物研究所,山西太原 0300002. 福建农林大学作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室,福建福州 3500023. 福建农林大学农业农村部东南黄红麻实验观测站/福建省麻类种质资源共享平台,福建福州 350002 |
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| 基金项目： | 国家自然科学基金项目(31771369);山西农科院种业发展专项(2016zyzx6);福建省作物设计育种重点实验室开放基金项目(2019zwsjyz003) |
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| 摘 要： | 大麻是雌雄异株的异花授粉作物,在苗期鉴定出植株的性别,对大麻育种及生产具有重要的意义。为开发与籽用大麻品种性别连锁的SCAR标记,本研究以汾麻3号、榆社麻和苍山麻为试验材料,研究已有的11个性别连锁标记在籽用大麻品种中的适用性。结果表明,3个标记（OPD-05、UBC-354和MADC2）在籽用大麻品种中呈雄性特异,尚未发现雌性特异条带。回收雄性特异条带并测序,根据测序结果新开发了6个SCAR标记,其中MADC2-8表现最好;进而利用123份已知性别的样本来检测MADC2-8的准确性,发现其平均准确性高达98.34%。该研究不仅丰富了大麻性别鉴定的分子标记,而且为大麻性别研究和实际应用奠定基础。
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| 关 键 词： | 大麻 籽用品种 性染色体 SCAR MADC |
| 收稿时间： | 2019-04-17 |
Evaluation of Markers Linked to Sex-specific and Development of SCAR Makers in Seed Hemp (Cannabis sativa L.) |
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| ZHAO Mingsen,FANG Shusheng,CHEN Yao,KANG Hongmei,GAO Jinhu,CHEN Siyuan,YANG Xin,FENG Xuping,ZHANG Liwu.Evaluation of Markers Linked to Sex-specific and Development of SCAR Makers in Seed Hemp (Cannabis sativa L.)[J].Chinese Journal of Tropical Crops,2019,40(10):2076-2082. |
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| Authors: | ZHAO Mingsen FANG Shusheng CHEN Yao KANG Hongmei GAO Jinhu CHEN Siyuan YANG Xin FENG Xuping ZHANG Liwu |
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| Abstract: | Hemp (Cannabis sativa L.) is a dioecious and cross pollinated plant. Sex identification of plants at seedling stage is of great significance for breeding and production in hemp. To develop new SCAR markers linked to sex-specific, three Chinese local seed hemp varieties, Fenma No.3, Yushema and Cangshanma, were used as the experimental materials to investigate the application of 11 markers linked to sex-specific in Chinese local seed hemp varieties in this study. The results showed that three makers (OPD-05 and UBC-354 as well as MADC2) were male-specific, while no female specific bands were found. Novel SCAR markers were developed on the basis of sequences of male specific bands. Among them, MADC2-8 was the best. Furthermore, 123 samples with known sex identification were used to assess the accuracy of MADC2-8. The average accuracy was 98.34%. These results would not only enrich the molecular markers linked to sex-specific, but also lay a foundation for the research and practical application of sex identification in hemp. |
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| Keywords: | Cannabis sativa seed variety sex chromosome SCAR MADC |
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