| 农杆菌注射法瞬时转化荔枝果实组织的研究 |
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| 引用本文： | 王树军,王果,李焕苓,孙进华,李芳,王家保.农杆菌注射法瞬时转化荔枝果实组织的研究[J].热带作物学报,2019,40(12):2405-2410. |
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| 作者姓名： | 王树军 王果 李焕苓 孙进华 李芳 王家保 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,海南海口 571101 |
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| 基金项目： | 海南省自然科学基金(No.319QN270);国家现代农业产业技术体系(No.CARS-32-03) |
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| 摘 要： | 为了寻找一种高效的荔枝果实瞬时基因表达方法,本研究以荔枝品种‘新球蜜荔’（Litchi chinensis Sonn. var. ‘Xinqiumili’）为试材,利用农杆菌注射法对荔枝果实组织进行转化,研究了果实发育时期、菌株种类、注射部位、取样时间、菌液浓度等对转化效率的影响。结果表明：选择果肉已完全包裹种子的Ⅱb期果实进行连体注射,在果柄、果皮、种子、果肉分别注入OD600值为2.4的农杆菌菌株GV3101,4 d后取样进行检测,4个组织的GUS染色率较高。本研究成功建立了适用于荔枝果实的基因瞬时表达系统,为今后快速鉴定荔枝果实相关基因功能提供了基础。
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| 关 键 词： | 农杆菌注射 ‘新球蜜荔’果实 瞬时表达 GUS检测 |
| 收稿时间： | 2019-05-20 |
Transformation of Tissues of Litchi Fruit with Injection of Agrobacterium |
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| WANG Shujun,WANG Guo,LI Huanling,SUN Jinhua,LI Fang,WANG Jiabao.Transformation of Tissues of Litchi Fruit with Injection of Agrobacterium[J].Chinese Journal of Tropical Crops,2019,40(12):2405-2410. |
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| Authors: | WANG Shujun WANG Guo LI Huanling SUN Jinhua LI Fang WANG Jiabao |
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| Institution: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | To find an efficient transient gene expression method for litchi, the fruits of ‘Xinqiumili’ were used as the material to transform fruit tissues by Agrobacterium in this study. The effects of fruit developing stage, bacterium strains, injection sites, sampling time, bacteria solution concentration on the transformation efficiency were studied. The results showed that the GUS staining rates were better in four tissues under the condition that Agrobacterium strain GV3101 with OD600 of 2.4 was injected into the stalk, peel, seed and pulp of the stage Ⅱ b fruit with the pulp completely wrapped in the seed in vivo, and the fruits were sampled for detection after four days of injection. The transient gene expression system of litchi fruits was successfully established, which laid a foundation for the rapid identification of related genes of litchi fruits in the future. |
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| Keywords: | injection of Agrobacterium ‘Xinqiumili’ fruit transient expression GUS detection |
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