| 2种氧弹热量计测定样品总能的差异及其对能量消化率的影响 |
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| 引用本文： | 杜中原,苏艳芳,陈凯旋,张虎,高庆涛,赵峰.2种氧弹热量计测定样品总能的差异及其对能量消化率的影响[J].动物营养学报,2021,33(2). |
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| 作者姓名： | 杜中原 苏艳芳 陈凯旋 张虎 高庆涛 赵峰 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193 |
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| 基金项目： | 中国农业科学院科技创新工程(ASTIP-IAS07);温氏食品集团股份有限公司-中国农业科学院北京畜牧兽医研究所合作项目(2018-YF-01)。 |
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| 摘 要： | 本试验旨在研究2种氧弹热量计测定样品总能(GE)的差异及其对能量消化率的影响。采用两样本比较试验设计,考察2种氧弹热量计测定4个饲粮、3个饲料原料及4个粪样的GE与其变异系数的差异,以及测定饲粮、饲料原料和仿生消化未消化残渣GE后计算的酶水解物能值(EHGE)和能量消化率的差异。在GE日内与日间变异的测定中,在每天09:00—21:00,每3 h测定1次样品的GE值,共测定4次,累计测定4 d。在仿生消化法饲粮能量消化率的测定中,每个样品的仿生消化设10个重复,未消化残渣绝干后随机分成2组。2种氧弹热量计测定饲粮或饲料原料GE,每个样品重复测定2次,对每个未消化残渣重复样品的GE测定1次。结果表明:1)2种氧弹热量计测定饲粮、饲料原料的GE存在显著差异(P<0.01),而粪样的GE差异不显著(P>0.05)。2)与氧弹热量计型号1相比,氧弹热量计型号2测定粪样GE的日内、日间及总变异系数相对较小。3)2种氧弹热量计测定样品及残渣的GE,由此计算的饲粮3、棉籽粕的EHGE在2种氧弹热量计之间有显著差异(P<0.05),饲粮3及3个饲料原料的能量消化率在2种氧弹热量计之间有显著差异(P<0.05)。由此可见,2种型号氧弹热量计在测定饲粮及饲料原料的GE上存在差异,在测定粪便GE的重复性上差异较大。2种氧弹热量计间测定样品GE的差异引起了部分样品EHGE及能量消化率的差异,但这一差异程度远低于GE差异的程度。
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| 关 键 词： | 氧弹热量计 饲料原料 粪样 总能 能量消化率 |
Difference in Two Types of Oxygen Bomb Calorimeter to Determine Gross Energy and Its Effects on Energy Digestibility of Samples |
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| DU Zhongyuan,SU Yanfang,CHEN Kaixuan,ZHANG Hu,GAO Qingtao,ZHAO Feng.Difference in Two Types of Oxygen Bomb Calorimeter to Determine Gross Energy and Its Effects on Energy Digestibility of Samples[J].Acta Zoonutrimenta Sinica,2021,33(2). |
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| Authors: | DU Zhongyuan SU Yanfang CHEN Kaixuan ZHANG Hu GAO Qingtao ZHAO Feng |
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| Institution: | (State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | The objective of this experiment was to investigate the difference in the two types of oxygen bomb calorimeter to determine gross energy(GE)and its effects on energy digestibility of samples.A two-sample comparison experimental design was adopted to study the difference in GE and its coefficient of variation for four diets,three feed ingredients and four feces determined with two types of oxygen bomb calorimeters,as well as the difference in the enzymatic hydrolysate gross energy(EHGE)and energy digestibility calculated from the GE of diets,feed ingredients and corresponding residues after simulated digestion.In the determination of intra-day and inter-day variation,the GE of the samples were measured four times daily with once every 3 h from 09:00 to 21:00 and continued to measure for 4 d.In the determination of diet energy digestibility in the simulated digestion,each sample contained 10 replicates.The undigested residues were dried and randomly divided into 2 groups.The two types of oxygen bomb calorimeter were used to measure the GE of each diets or feed ingredients in duplicate,and the GE of each undigested residues only once.The results showed as follows:1)there were significant differences between the GE measured by the two types of oxygen bomb calorimeter for each of diets and feed ingredients(P<0.05),while no difference was observed between the GE of feces.2)The smaller intra-day,inter-day and the total coefficients of variation of GE for feces were observed in the oxygen bomb calorimeter type 2 compared with oxygen bomb calorimeter type 1.3)There were significant differences in the EHGE in each of diet 3 and cottonseed meal and energy digestibilities in each of diet 3 and three feed ingredients calculated from the GE of samples and undigested residues measured by two types of oxygen bomb calorimeter(P<0.05).In conclusion,two types of oxygen bomb calorimeter present a difference in GE measurement for diets and feed ingredients,and present a great difference in the repeatability of fecal GE.Differences in GE measured by two types of oxygen bomb calorimeter lead to differences in EHGE and energy digestibility in some samples,but the extent of this difference is much less than the difference in GE. |
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| Keywords: | oxygen bomb calorimeter feed ingredients feces gross energy energy digestibility |
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