| 三七素提取工艺及检测方法的优化 |
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| 引用本文： | 段绍凤,陈庚,闫静,覃忠明,张广辉,梁艳丽.三七素提取工艺及检测方法的优化[J].热带作物学报,2019,40(11):2255-2260. |
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| 作者姓名： | 段绍凤 陈庚 闫静 覃忠明 张广辉 梁艳丽 |
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| 作者单位： | 1. 云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,云南昆明 6502012. 云南农业大学云南省药用植物生物学重点实验室,云南昆明 6502013. 云南农业大学农学与生物技术学院,云南昆明 650201 |
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| 基金项目： | 国家自然科学基金项目(8176140700) |
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| 摘 要： | 本研究以三七（Panax notoginseng）植株及其产品和近缘种为研究对象,采用L9(3 4)正交试验对三七素的提取工艺进行了优化,然后利用HPLC法检测三七素含量,并对各样品中的三七素含量进行了比较分析。结果表明：优化后的三七素提取条件为超纯水、料液比1∶20（g/mL）,提取1次（10 min）;三七素在三七花蕾中的含量最高,其次是根状茎和主根,但是三七茎叶中也有较高含量的三七素,说明三七花蕾和茎叶均可作为三七素的提取原料;在三七产品中三七花的三七素含量最高,达到3.33%,其次是剪口（根状茎）、筋条（侧根）和须根;但三七素的含量与三七个头大小（头数）并无明显的相关性;在三七近缘种姜状三七（P. zingiberensis）和野三七（P. vienamensis var. fuscidiscus）主根、珠子参（P. japonicus var. major）根状茎中的三七素含量显著低于三七主根三七素含量,也显著低于三七其他部位的三七素含量,说明三七是最佳的三七素来源植物。三七素的含量主要受到物种和部位的影响,与产品规格无关。本文所建立的三七素提取方法和HPLC检测方法,能够快速简便、稳定可靠测定样品中三七素含量;并可为三七素相关标准的建立奠定基础,为三七的综合利用开发提供依据。
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| 关 键 词： | 三七 三七素 HPLC 超声提取 |
| 收稿时间： | 2019-02-19 |
Optimization of Extraction Process and Detection Method of Dencichine |
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| DUAN Shaofeng,CHEN Geng,YAN Jing,QIN Zhongming,ZHANG Guanghui,LIANG Yanli.Optimization of Extraction Process and Detection Method of Dencichine[J].Chinese Journal of Tropical Crops,2019,40(11):2255-2260. |
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| Authors: | DUAN Shaofeng CHEN Geng YAN Jing QIN Zhongming ZHANG Guanghui LIANG Yanli |
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| Institution: | 1. National & Local Joint Engineering Research Center on Germplasms Utilization & Innovation of Chinese Medicinal Materials in Southwest, Yunnan Agricultural University, Kunming, Yunnan 650201, China2. Yunnan Provincial Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming, Yunnan 650201, China3. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan 650201, China |
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| Abstract: | The extraction process of dencichine in Panax notoginseng was optimized by the L9(3 4) orthogonal test, and the content of dencichine was determined by HPLC. The optimized extraction condition for dencichine was extracted once with ultrapure water at solid-to-liquid ratio of 1∶20 (g/mL) for 10 min. Flower bud had the highest dencichine content, followed by rhizome, main roots and leaves. The content of dencichine in flower buds was 3.33%. There was no significant correlation between the content of dencichine and the rhizom size. The content of dencichine in the rhizome of P. zingiberensis and P. vienamensis var. fuscidiscus was significantly lower than that of the main root of P. notoginseng. It was also significantly lower than that of other parts of P. notoginseng, indicating that P. notoginseng was the best source of dencichine. The content of dencichine was mainly affected by species and parts, and had nothing to do with product specifications. The extraction method and HPLC detection method established in this paper could quickly and easily determine the content of dencichine. It can lay a foundation for the establishment of related standards of dencichine and provide a basis for the comprehensive utilization and development of P. notoginseng. |
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| Keywords: | Panax notoginseng dencichine HPLC supersonic extraction |
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