| 荔枝果皮MADS-box基因的克隆与初步表达分析 |
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| 引用本文： | 肖 靖,赖 彪,赵志常,秦永华,胡桂兵.荔枝果皮MADS-box基因的克隆与初步表达分析[J].热带作物学报,2012,33(3):439-445. |
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| 作者姓名： | 肖 靖 赖 彪 赵志常 秦永华 胡桂兵 |
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| 作者单位： | 华南农业大学园艺学院,广东广州510642;亚热带农业生物资源保护与利用国家重点实验室,广东广州 510642 |
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| 基金项目： | 现代农业产业技术体系建设专项资金（No. nycytx-32）；国家自然科学基金项目（No. 30971985）。 |
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| 摘 要： | 采用RT-PCR结合RACE技术从‘糯米糍’荔枝果皮中克隆到了1个1 208 bp的MADS-box基因，命名为LcMADS9。该基因包含1个738 bp的完整的开放阅读框, 编码245个氨基酸，具有典型的MADS-box基因结构，其编码的蛋白与其它植物的MADS-box蛋白有较高的一致性，其中与欧洲白桦（Betula pendula）的同源性高达80％。系统发育树分析结果表明，LcMADS9基因属于MADS-box基因家族中AP1/SQUA-like亚家族。实时荧光定量PCR分析结果表明，该基因在叶片、果皮和花中均有表达，而在根、茎和果肉中几乎没有表达。
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| 关 键 词： | 荔枝 果皮 MADS-box 克隆 表达分析 |
Cloning and Expression of MADS-box Gene from Litchi(Litchi chinensis Sonn.)Pericarps |
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| XIAO Jing,LAI Biao,ZHAO Zhichang,QIN Yonghua and HU Guibing.Cloning and Expression of MADS-box Gene from Litchi(Litchi chinensis Sonn.)Pericarps[J].Chinese Journal of Tropical Crops,2012,33(3):439-445. |
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| Authors: | XIAO Jing LAI Biao ZHAO Zhichang QIN Yonghua and HU Guibing |
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| Institution: | 1 Collage of Horticulture, South China Agricultural University
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University;1 Collage of Horticulture, South China Agricultural University
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University;1 Collage of Horticulture, South China Agricultural University
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University;1 Collage of Horticulture, South China Agricultural University
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University;1 Collage of Horticulture, South China Agricultural University
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University |
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| Abstract: | A MADS-box gene,which is 1 208 bp long named LcMADS9,was cloned from the pericarp of ’Nuomici’litchi cultivar by using RT-PCR and RACE technique.This gene contained an ORF of 738 bp encoding 245 putative amino acid residues,had the typical MADS-box genetic structure.Homology analysis of putative amino acid residues showed that LcMADS9 had high homology to other MADS-box proteins from different species,especially 80% similarity to Betula pendula.Phylogenetic analysis indicated that LcMADS9 belonged to a subfamily AP1/SQUA-like of the MADS-box.LcMADS9 was expressed by the real-time PCR analysis in leaves,pericarps and flowers,almost no expression in roots,stems and flesh. |
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| Keywords: | Litchi Pericarp MADS-box Cloning Expression analysis |
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