| 荔枝种内原生质体电融合参数研究 |
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| 引用本文： | 马新业,曾继吾,易干军.荔枝种内原生质体电融合参数研究[J].热带作物学报,2012,33(6):1030-1033. |
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| 作者姓名： | 马新业 曾继吾 易干军 |
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| 作者单位： | 1. 广州中医药大学中药资源科学与工程研究中心,广东广州,510006
2. 广东省农业科学院果树研究所,广东广州,510640 |
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| 摘 要： | 选择“桂味”和“妃子笑”两个荔枝(Litchi chinensis Sonn.)品种的花药胚性细胞悬浮系来源的原生质体作融合亲本,在优化电融合参数(排列电压5V,脉冲电压80V,脉冲持续时间45～65 μs,原生质体密度5.0×105～7.5×105个/mL,融合液Ca2+浓度0.2 mmol/L,甘露醇浓度10％～11％)条件下,可获得20％以上双元异核融合率.在亲本原生质体混合前,以0.02％中性红10 min染色“桂味”原生质体,有助于双元异核融合体的有效确认.
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| 关 键 词： | 荔枝 原生质体融合 中性红 |
Optimization of the Parameters on Protoplast Electrofusion of Lychee |
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| MA Xinye,ZENG Jiwu and YI Ganjun.Optimization of the Parameters on Protoplast Electrofusion of Lychee[J].Chinese Journal of Tropical Crops,2012,33(6):1030-1033. |
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| Authors: | MA Xinye ZENG Jiwu and YI Ganjun |
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| Institution: | Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Traditional Chinese Medicine;Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences;Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences |
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| Abstract: | The protoplasts of lychee(Litchi chinensis Sonn.)cv.Guiwei and cv.Feizixiao isolated respectively from cell suspension cultures from anther-derived calli were used for the fusion.Above 20% binuclear heterokaryons frequency was achieved under following conditions: alternate current field strength of 5 V,direct current pulse strength of 80 V,pulse duration of 45～65 μs,protoplast density at 5.0×105～7.5×105 per mL,0.2 mmol/L Ca2+ and 10%～11% mannitol in electrical fusion solution.Binuclear heterokaryons frequency could be easily determined by detaining patent protoplast of lychee cv.Guiwei with 0.02% netural red for 10 min before fusing.The results could be a reference for large-scale electrofusing and subsequent hybrid screening of litchi protoplast. |
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| Keywords: | Lychee(Litchi chinensis Sonn ) Netural red Protoplast electrofusion |
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