| 香蕉后熟果皮差异蛋白分析及其基因克隆的研究 |
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| 引用本文： | 李艳霞,冯仁军,张丽丽,张银东.香蕉后熟果皮差异蛋白分析及其基因克隆的研究[J].热带作物学报,2012,33(6):1057-1062. |
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| 作者姓名： | 李艳霞 冯仁军 张丽丽 张银东 |
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| 作者单位： | 1. 海南大学农学院,海南海口,570228
2. 海南大学农学院,海南海口 570228;中国热带农业科学院热带作物生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室,海南海口 571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费项目“乙烯诱导的香蕉果皮差异表达蛋白的研究”（No. ITBB110204）。 |
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| 摘 要： | 提取乙烯诱导香蕉后熟初期的果皮总蛋白和对照果皮总蛋白,进行双向电泳实验,利用ImageMaster软件找出乙烯诱导的香蕉果皮中的差异蛋白点,并且对差异蛋白进行质谱分析,选取一个可能为Ⅲ类酸性几丁质酶(MaCHⅢ)的蛋白进行后续研究.利用MaCHⅢ的肽段信息在NCBI上比对后找到与该蛋白相应的核酸信息,设计PCR反应引物,扩增MaCHⅢ基因,得到DNA全长序列.结果表明,该基因与Ⅲ类酸性几丁质酶的同源性为99％,可以初步确定这个基因为Ⅲ类酸性几丁质酶基因.
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| 关 键 词： | 香蕉 后熟 乙烯诱导 差异蛋白 几丁质酶基因 |
Analysis of Differentially Expressed Proteins and the Gene Cloning in Ripening Banana Peel |
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| LI Yanxi,FENG Renjun,ZHANG Lili and ZHANG Yindong.Analysis of Differentially Expressed Proteins and the Gene Cloning in Ripening Banana Peel[J].Chinese Journal of Tropical Crops,2012,33(6):1057-1062. |
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| Authors: | LI Yanxi FENG Renjun ZHANG Lili and ZHANG Yindong |
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| Institution: | 1,2 1 College of Agronomy,Hainan University,Haikou,Hainan 570228,China 2 Key Laboratory of the Ministry of Agriculture,Chinese Academy of Tropical Agricultural Sciences, Institute of Tropical Biosciences and Biotechnology,Haikou,Hainan 571101,China |
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| Abstract: | Differentially expressed proteins were isolated by the two-dimensional(2D)gel electrophoresis,and analysed by the Image Master software,and identified by the mass spectrometry,after the extraction of the total protein of the control and the ethylene-induced early-ripening banana peel.A putative chitinase Ⅲ(MaCHⅢ)was picked out for subsequent research from the differentially expressed proteins.The nucleic acid sequence to corresponding peptide sequence of MaCHⅢ was found by the NCBI BLAST.The full-length DNA sequence of MaCHⅢ gene was obtained by polymerase chain reaction(PCR)using PCR primers,which were designed according to the nucleic acid sequence.The results showed that this sequence share about 99% homology to a Ⅲ class acidic chitinase in other species.And then,it was preliminarily confirmed as a gene of Ⅲ kind of acidic chitinase in banana. |
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| Keywords: | Banana Ripening Ethylene-induced Differentially expressed proteins Chitinase gene |
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