| Diversity and Characterization of Potential H2-Dependent Fe(Ⅲ)-Reducing Bacteria in Paddy Soils |
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| 作者姓名： | LI Hui-Juan PENG Jing-Jing LI Hong-Bo |
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| 作者单位： | Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China(No.41090282). |
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| 摘 要： | Microbial ferric iron reduction, with organic carbon or hydrogen as the electron donor, is one of the most important biogeochemical processes in anoxic paddy soils; however, the diversity and community structure of hydrogen-dependent dissimilatory iron-reducers remain unknown. Potential H2-dependent Fe(III)-reducing bacteria in paddy soils were explored using enrichment cultures with ferrihydrite or goethite as the electron acceptor and hydrogen as the electron donor. Terminal restriction fragment length polymorphism (T-RFLP) analysis and cloning/sequencing were conducted to reveal bacterial community structure. Results showed that Geobacter and Clostridium were the dominant bacteria in the enrichment cultures. Fe(III) oxide mineral phases showed a strong effect on the community structure; Geobacter and Clostridium were dominant in the ferrihydrite treatment, while Clostridium spp. were dominant in the goethite treatment. These suggested that H2-dependent Fe(III)-reducing bacteria might be widely distributed in paddy soils and that besides Geobacter, Clostridium spp. might also be an important group of H2-dependent Fe(III)-reducing microorganisms.
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| 关 键 词： | bacterial community structure Clostridium enrichment culture Geobacter terminal restriction fragment length poly-morphism |
| 收稿时间： | 2011/4/12 0:00:00 |
| 修稿时间： | 2012/7/18 0:00:00 |
Diversity and characterization of potential H2-dependent Fe(III)-reducing bacteria in paddy soils |
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| LI Hui-Juan,PENG Jing-Jing,LI Hong-Bo.Diversity and characterization of potential H2-dependent Fe(III)-reducing bacteria in paddy soils[J].Pedosphere,2012,22(5):673-680. |
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| Authors: | LI Hui-Juan PENG Jing-Jing and LI Hong-Bo |
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| Institution: | Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Microbial ferric iron reduction, with organic carbon or hydrogen as the electron donor, is one of the most important biogeochemical processes in anoxic paddy soils; however, the diversity and community structure of hydrogen-dependent dissimilatory iron-reducers remain unknown. Potential H2-dependent Fe(III)-reducing bacteria in paddy soils were explored using enrichment cultures with ferrihydrite or goethite as the electron acceptor and hydrogen as the electron donor. Terminal restriction fragment length polymorphism (T-RFLP) analysis and cloning/sequencing were conducted to reveal bacterial community structure. Results showed that Geobacter and Clostridium were the dominant bacteria in the enrichment cultures. Fe(III) oxide mineral phases showed a strong effect on the community structure; Geobacter and Clostridium were dominant in the ferrihydrite treatment, while Clostridium spp. were dominant in the goethite treatment. These suggested that H2-dependent Fe(III)-reducing bacteria might be widely distributed in paddy soils and that besides Geobacter, Clostridium spp. might also be an important group of H2-dependent Fe(III)-reducing microorganisms. |
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| Keywords: | bacterial community structure Clostridium enrichment culture Geobacter terminal restriction fragment length polymorphism |
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