| 蓖麻基因组中一种Mutator-lik类型MITEs元件的结构分析和鉴定 |
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| 引用本文： | 周新成,王海燕,胡梅珍,李正绪,卢诚,王文泉.蓖麻基因组中一种Mutator-lik类型MITEs元件的结构分析和鉴定[J].热带作物学报,2012,33(2):327-331. |
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| 作者姓名： | 周新成 王海燕 胡梅珍 李正绪 卢诚 王文泉 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室,海南海口,571101 |
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| 基金项目： | 国家973项目课题“木薯淀粉高效累积途径及其关键基因”（2010CB126601）；国家木薯产业技术体系生物技术岗（CARS-12）。 |
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| 摘 要： | 利用生物信息学方法，对蓖麻基因组Mutator-like类型MITEs元件进行了分析和鉴定。结果表明，蓖麻基因组上共鉴定出76个小于3 kb的Mutator-like类型MITEs元件，其中长度小于1 000 bp的元件有53个，占69.7％。这些MITEs元件中有42个位于基因间隔区，11个位于基因内含子区，位于5'UTR和3'UTR区的分别有8个和4个，另有1个位于基因编码区。29个MITEs元件带有转座酶以外的功能基因片段，成为pack-MITEs。多个pack-MITEs带有相同的非转座酶基因片段。Mutator-like类型的MITEs对蓖麻基因的表达和基因组的进化有潜在的影响，值得深入分析。
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| 关 键 词： | MITEs Mutator-like 蓖麻 转座子 |
Identification and Characterization of Mutator-like MITEs in Castor Bean Genome |
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| ZHOU Xincheng,WANG Haiyan,HU Meizhen,LI Zhengxu,LU Cheng and WANG Wenquan.Identification and Characterization of Mutator-like MITEs in Castor Bean Genome[J].Chinese Journal of Tropical Crops,2012,33(2):327-331. |
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| Authors: | ZHOU Xincheng WANG Haiyan HU Meizhen LI Zhengxu LU Cheng and WANG Wenquan |
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| Institution: | Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture / Institute of
Tropical Bioscience and Biotechnology, CATAS;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture / Institute of
Tropical Bioscience and Biotechnology, CATAS;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture / Institute of
Tropical Bioscience and Biotechnology, CATAS;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture / Institute of
Tropical Bioscience and Biotechnology, CATAS;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture / Institute of
Tropical Bioscience and Biotechnology, CATAS;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture / Institute of
Tropical Bioscience and Biotechnology, CATAS |
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| Abstract: | Mutator-like MITEs were identified and characterized from castor bean(Ricinus communis L.)genome using bioinformatics methods.There were seventy-six Mutator-like MITEs less than 3 kb in length in castor bean,of which 53 elements,about 69.7% in total,being less than 1000 bp.Forty-two MITEs were located in intergenic regions,11 in introns and 8 and 4 elements in 5’ UTR and 3’ UTR regions,respectively.Only one element was inserted in the coding region of gene.Twenty-nine pack-MITEs were found containing gene fragment sequences unrelated to transposase gene.And some pack-MITEs carried with the sequence fragments belonging to the same gene.The results showed that Mutator-like MITEs may play potential roles in the regulation of gene expression and genome evolution in castor bean. |
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| Keywords: | MITEs Mutator-like Castor bean Ricinus communis L Transposon |
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