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| 华仁杏杂种鉴定及遗传变异分析 | |
| 引用本文： | 刘梦培,傅大立,李芳东,傅建敏,田敏,梁臣.华仁杏杂种鉴定及遗传变异分析[J].林业科学研究,2012,25(1):88-92. |
| 作者姓名： | 刘梦培 傅大立 李芳东 傅建敏 田敏 梁臣 |
| 作者单位： | 1. 中国林业科学研究院经济林研究开发中心,河南郑州,450003 2. 中国林业科学研究院经济林研究开发中心,河南郑州450003;北京林业大学,北京100083 3. 中国林业科学研究院亚热带林业研究所,浙江富阳,311400 4. 河南省洛阳林业科学研究所,河南洛阳,471002 |
| 基金项目： | 国家十一五科技支撑项目资助(2006BAD01A1703-2) |
| 摘 要： | 以华仁杏6个杂交组合为实验材料,利用SSR分子标记技术研究亲本与杂种扩增谱带的多态性,以甄别真假杂种。筛选出在亲本和杂种之间存在双亲互补带型的引物6对,除2个杂种无法鉴别外,其余品种均可鉴别为真杂种。另外亲缘关系和遗传变异分析结果表明,各个组合杂交后代表现出来的一致性较高,遗传多样性水平相差不大,但当父本选择X1时,母本选择H1或H3杂交后代差异比较显著。 |
| 关 键 词： | 华仁杏 SSR 杂种鉴定 聚类分析 遗传变异 |
| 收稿时间： | 4/7/2011 12:00:00 AM |
Identification and Genetic Variation Analysis of Armeniaca cathayana Hybrids |
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| LIU Meng-pei,FU Da-li,LI Fang-dong,FU Jian-min,TIAN Min and LIANG Chen.Identification and Genetic Variation Analysis of Armeniaca cathayana Hybrids[J].Forest Research,2012,25(1):88-92. | |
| Authors: | LIU Meng-pei FU Da-li LI Fang-dong FU Jian-min TIAN Min and LIANG Chen |
| Institution: | Non-timber Forestry Research and Development Center,Chinese Academy of Forestry,Zhengzhou 450003,Henan,China;Non-timber Forestry Research and Development Center,Chinese Academy of Forestry,Zhengzhou 450003,Henan,China; Beijing Forestry University,Beijing 100083,China;Non-timber Forestry Research and Development Center,Chinese Academy of Forestry,Zhengzhou 450003,Henan,China;Non-timber Forestry Research and Development Center,Chinese Academy of Forestry,Zhengzhou 450003,Henan,China;Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400,Zhejiang,China;Research Institute of Forestry in Luoyang,Luoyang 471002,Henan,China |
| Abstract: | With six hybridized combinations of Armeniaca cathayana as experimental materials, the SSR molecular marker technology was used to identify hybrids through amplification polymorphism bands and 6 primers with parent complementary belt type were screened. Most of the hybrids were identified as true hybrids except two. In addition, the genetic relationship and genetic variation analysis results showed that all the hybrid combination displayed high consistency and not significant genetic diversity was found. When choosing X1 as male parent and H1 or H3 as female parent, the differences of hybrids were significant. The results of hybrid identification and genetic variation analysis can be used in future's hybrid parents breeding, genetic linkage map construction and QTL mapping of important character of A. cathayana. |
| Keywords: | Armeniaca cathayana SSR hybrid identification clustering analysis genetic variation |
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