| 桃褐腐病菌的拮抗细菌CE的发酵条件研究 |
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| 引用本文： | 赵晓燕,刘正坪,武硕,杨海清,刘素花.桃褐腐病菌的拮抗细菌CE的发酵条件研究[J].中国农学通报,2008,24(10):413-418. |
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| 作者姓名： | 赵晓燕 刘正坪 武硕 杨海清 刘素花 |
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| 作者单位： | 北京农学院植物科学技术系,北京,102206 |
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| 基金项目： | 北京市教委资助项目，北京市农委资助项目，北京市属市管高校人才强教计划 |
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| 摘 要： | 通过单因素及正交试验确定了桃褐腐病菌的拮抗细菌CE的最佳发酵液的组成和发酵的最佳条件。实验结果表明：发酵液中的C、N源的组合、C、N源的含量、金属离子对拮抗细菌CE的生长量和抑菌活性都有不同的影响，用4.5%的玉米粉和0.15%的酵母浸膏配制的发酵液对拮抗细菌CE生长量和抑菌效果都相对较好，另外Fe3+的添加能同时促进拮抗细菌的生长量和抑菌效果。在研究的几个发酵条件中，拮抗细菌CE的生长量和抑菌效果在一定范围内成正相关，即随生长量增大抑菌效果增强，说明抑菌物质的产生与活菌量的多少有关，最适的发酵条件为30℃，pH7，140r/min，接种量为10%，发酵时间为60h。
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| 关 键 词： | 桃褐腐病菌 拮抗细菌 发酵条件 |
| 收稿时间： | 2008-06-17 |
| 修稿时间： | 2008-08-06 |
Fermentation Conditions for Antagonistic Bacterium CE against Monilinia fruticola |
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| Zhao Xiaoyan,Wu Shuo,Yang Haiqing,Liu Sulnua,Liu Zhengping.Fermentation Conditions for Antagonistic Bacterium CE against Monilinia fruticola[J].Chinese Agricultural Science Bulletin,2008,24(10):413-418. |
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| Authors: | Zhao Xiaoyan Wu Shuo Yang Haiqing Liu Sulnua Liu Zhengping |
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| Abstract: | The composition of fermented broth and fermentation conditions were optimized by monofactorial and orthogonal experiment. Combination and content of the C and N sources, as well as mental ions, have different effect on the growth and antagonistic effect of bacterium CE. The broth composed of 4.5% corn flour and 0.15% yeast extract is better for both growth and antagonistic effect of CE. Adding of Fe3+ can promote both growth and antagonistic effect. The antagonistic effect of bacterium CE is correlation with growth under the studied conditions in a certain extent, that is, the antagonistic effect is increased with the growth of CE. The most preferable condition is 30℃, pH7, 140r/min, 10% inoculation, and fermented for 60h. |
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| Keywords: | Monilinia fruticolazz Antagonistic bacteriumzz Fermentation conditionszz |
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