Cryopreservation of sperm from spotted wolffish |
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| Authors: | Snorri Gunnarsson Sindri Sigurdsson Helgi Thorarensen Albert K Imsland |
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| Institution: | (1) Akvaplan-niva Iceland Office, Akralind 4, 201 Kópavogur, Iceland;(2) Hlyri ehf, Hafnarbraut 6, 740 Neskaupstadur, Iceland;(3) Holar Agricultural University, Holar i Hjaltadal, 551 Saudarkrokur, Iceland;(4) Department of Biology, University of Bergen, Bergen, Norway |
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| Abstract: | The effects of different concentrations of cryoprotectant (dimethyl sulfoxide; DMSO), cooling rate and straw size on the post-thaw
motility of frozen sperm from spotted wolffish, Anarhichas minor, were studied. There was no significant difference in the post-thaw motility of sperm treated with three different concentrations
of DMSO (10, 20 and 30%). Similarly, there was no significant difference in the post-thaw motility of spermatozoa when using
different freezing rates (i.e. distance of straws from the surface of liquid N2, 4.7, 5.5 and 7.1°C min−1) and the straw size (0.5 and 1.0 ml) did not affect survival. The cryopreservation of sperm can be used to make up for the
frequent lack of sperm and/or the unsynchronised timing of sperm production in spotted wolffish males and the ovulation time
in females. The results show that sperm from spotted wolffish can be frozen to secure access to viable sperm, but further
experiments are needed in order to reveal the effect of different parameters on the post-thawing mortality and define the
optimum conditions for cryopreservation. |
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| Keywords: | Cryopreservation Sperm Post-thaw motility Spotted wolffish (Anarhichas minor Olafsson 1772) |
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