| EGCG-O-甲基转移酶(EOMT)在重组大肠杆菌中的表达条件研究 |
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| 引用本文： | 费冬梅,林智,吕海鹏,张悦,谭俊峰,郭丽.EGCG-O-甲基转移酶(EOMT)在重组大肠杆菌中的表达条件研究[J].茶叶科学,2011,31(4):333-340. |
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| 作者姓名： | 费冬梅 林智 吕海鹏 张悦 谭俊峰 郭丽 |
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| 作者单位： | 1. 中国农业科学院茶叶研究所/农业部茶及饮料作物产品加工与质量控制重点实验室,浙江 杭州 310008; 2. 中国农业科学院研究生院,北京 100081 |
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| 基金项目： | 国家自然科学基金项目,浙江省自然科学基金项目,现代农业产业技术体系建设专项资金项目 |
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| 摘 要： | 本研究以EGCG-O-甲基转移酶（EOMT）催化EGCG生成EGCG3"Me的产量为主要指标,探讨了重组大肠杆菌内EGCG-O-甲基转移酶的诱导表达条件。结果表明,当诱导剂IPTG终浓度为0.05mmol/L,诱导时间为20h,培养基初始pH为7.0,以及诱导温度为20℃时,EOMT的表达效果最佳。
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| 关 键 词： | EGCG-O-甲基转移酶 重组大肠杆菌 表达条件 |
| 收稿时间： | 2011-01-10 |
Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria |
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| FEI Dong-mei,LIN Zhi,LU Hai-peng,ZHANG Yue,TAN Jun-feng,GUO Li.Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria[J].Journal of Tea Science,2011,31(4):333-340. |
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| Authors: | FEI Dong-mei LIN Zhi LU Hai-peng ZHANG Yue TAN Jun-feng GUO Li |
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| Institution: | 1. Key Laboratory of Tea and Beverage Crops Processing and Quality Control, Ministry of Agriculture; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | EGCG3"Me could be produced from EGCG catalyzed by EGCG-O-Methyltransferase.Taken the yield of EGCG3"Me as main index,the present study focused on the producing conditions of EGCG-O-Methyltransferase induced by IPTG in recombinant E.coli bacteria.Results showed that the optimum producing conditions were as follows: the concentration of IPTG was 0.05 mmol/L,the induction time was 20 h,the initial pH of medium was 7.0 and the induction temperature was 20℃. |
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| Keywords: | EGCG-O-Methyltransferase recombinant Escherichia coli expression conditions |
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