| 对蓟马类害虫高致病性球孢白僵菌的分离、鉴定 |
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| 引用本文： | 王俊平,郑长英.对蓟马类害虫高致病性球孢白僵菌的分离、鉴定[J].茶叶科学,2011,31(4):295-299. |
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| 作者姓名： | 王俊平 郑长英 |
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| 作者单位： | 青岛农业大学农学与植物保护学院,山东青岛,266109 |
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| 基金项目： | 国家公益性行业(农业)科研专项经费项目，青岛农业大学高层次人才科研基金 |
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| 摘 要： | 从草履蚧僵虫体内分离出12株病原真菌,对蓟马类昆虫具有70%～90%的致死率.现选出一株病原真菌CYT10利用形态和分子的特点进行鉴定.扫描电镜下观察,该菌产孢细胞簇生瓶状,“之”型产孢轴.经PCR获得rDNA-ITS和EF1 -a核苷酸序列,与其他球孢白僵菌的相应序列一致性达95%以上,进化分析CYT10菌株与球孢白...
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| 关 键 词： | 球孢白僵菌 蓟马 形态特点 rDNA-ITS EF1-a |
Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips |
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| WANG Jun-ping,ZHENG Chang-ying.Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips[J].Journal of Tea Science,2011,31(4):295-299. |
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| Authors: | WANG Jun-ping ZHENG Chang-ying |
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| Institution: | * College of Agronomy and Plant Protection,Qingdao Agricultural university,Qingdao 266109,China |
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| Abstract: | 12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality.CYT10 strain was identified based on the morphological and molecular characteristics.Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced.The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana(>95%).Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain,which was belonging to B.bassiana.CYT10 strain was classified as a member of the species B.bassiana based on the morphological characteristics and analysis of DNA sequences. |
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| Keywords: | Beauveria bassiana thrip morphological characteristics rDNA-ITS EF1-a |
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