| 草鱼MyoD cDNA的克隆和序列分析 |
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| 引用本文： | 王立新,白俊杰,叶星,罗建仁,陈宏,简清,劳海华.草鱼MyoD cDNA的克隆和序列分析[J].中国农业科学,2005,38(10):2134-2138. |
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| 作者姓名： | 王立新 白俊杰 叶星 罗建仁 陈宏 简清 劳海华 |
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| 作者单位： | [1]西北农林科技大学动物科技学院／陕西省农业分子生物学重点实验室,杨凌712100 [2]中国水产科学研究院珠江水产研究所／中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州510380 [3]徐州师范大学生物技术研究所,徐州221116 |
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| 基金项目： | 中国水产科学研究院重点基金资助项目（99-08-02）. |
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| 摘 要： | 用RACE技术从受精后约22 h的草鱼胚胎总RNA中扩增获得了草鱼MyoD基因的全序列,并对其序列进行了分析。结果表明，草鱼MyoD基因全长cDNA为1 597 bp,其中开放阅读框为825 bp，共编码275个氨基酸，结构分析表明该肽链第1～84个氨基酸为草鱼MyoD基因的Basic区,第98～142个氨基酸为草鱼MyoD基因的HLH结构域，该序列所编码的肽链没有信号肽；通过对比分析已知的GeneBank中其它脊椎动物MyoD基因发现，该基因编码的氨基酸肽链随动物由低等向高等进化有加长的趋势,且核苷酸以及推测的氨基酸同源性和动物之间的亲缘关系相一致；草鱼MyoD基因的克隆为研究家鱼的肌肉发育调控的机理以及肉质改良奠定了基础。
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| 关 键 词： | 草鱼 MyoD基因 克隆 |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005-03-02 |
Molecular Coning and Sequence Analysis of MyoD in Grass Carp (Ctenopharyngodon idella) |
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| WANG Li-xin, BAI Jun-jie, YE Xing, LUO Jian-ren, CHEN Hong, JIAN Qing, LAO Hai-hua.Molecular Coning and Sequence Analysis of MyoD in Grass Carp (Ctenopharyngodon idella)[J].Scientia Agricultura Sinica,2005,38(10):2134-2138. |
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| Authors: | WANG Li-xin BAI Jun-jie YE Xing LUO Jian-ren CHEN Hong JIAN Qing LAO Hai-hua |
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| Institution: | 1.College of Animal Science and Technology, Northwest Science and Technology University of Agriculture and Forestry/Shaanxi Key Laboratory of Agricultural Molecular Biology; Yangling 712100; 2.Pearl River Fisheries Research Institute/Key Laboratory of Tropie and Subtropical Fish Breeding and Cultivation, Chinese Academy of Fishery Sciences, Guangzhou 510380; 3.Xuzhou Normal University, Institute of Bio-Cellular and Molecular, Xuzhou 221116 |
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| Abstract: | Total RNA was isolated from grass carp embryos at 22 h post-fertilization to clone the MyoD cDNA by using rapid cDNA end amplification, and the sequence was analyzed with other animals. The results showed that: the full-length of grass carp MyoD is 1 597 bp, with 825 bp open read frame enconding 275 amino acids, including a bHLH domain which composes of 1th to 84th amino acids and 98th to 141th amino acids, without signal peptide; Compared grass carp MyoD with other animals, the results showed that the length of MyoD peptide is related to the evolution of animal, and the homology of nucleotide and amino acid accords with genetic relationship among the animals; Cloning of grass carp MyoD is meaningful for researching the mechanism of fish's muscle development and dominate, it is also helpful for improving the muscle quality of fish artificially. |
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| Keywords: | Grass carp MyoD gene Cloning |
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