Molecular cloning of Insulin-like Growth Factor-I from triangular bream (Megalobrama terminalis) |
| |
| Authors: | Fudan Tong Hongyun Liu Qun Wang |
| |
| Institution: | (1) College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, P.R |
| |
| Abstract: | Triangular bream (Megalobrama terminalis) IGF-I DNA and gene were cloned from triangular bream liver for the first time by RT-PCR. Sequence analysis indicated that
the IGF-I cDNA consisted of 486 nucleotides encoding 117 amino acids which spanned the complete signal peptide, B, C, A, D
and E domains. Analysis of the E domain indicated that triangular bream IGF-I belongs to the IGF-I Ea-2 subtype. Compared
to bluntnose bream (Megalobrama amblycephala), another member of the same Megalobrama genus, triangular bream IGF-I shared 99.8% identity in cDNA sequence and 99.4% in predicted amino acid sequence. However,
considerable differences were found in comparison to with common carp (Cyprinus carpio) and grass carp (Ctenopharyngodon idellus), which are members of the same family but of a different genus.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
| Keywords: | carps IGF-I species differences triangular bream (Megalobrama terminalis) |
| 本文献已被 SpringerLink 等数据库收录！ |
|
