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| 虾青素合成关键酶基因bkt和CrtR-B双价植物表达载体的构建及对花生的遗传转化 | |
| 引用本文： | 徐静,杨庆利,檀琮萍,侯艳华,禹山林,秦松.虾青素合成关键酶基因bkt和CrtR-B双价植物表达载体的构建及对花生的遗传转化[J].河南农业科学,2007(12):35-39. |
| 作者姓名： | 徐静 杨庆利 檀琮萍 侯艳华 禹山林 秦松 |
| 作者单位： | 1. 淮海工学院,江苏省海洋生物技术重点建设实验室,江苏,连云港,222005;中国科学院,海洋研究所,山东,青岛266071 2. 山东省花生研究所,山东,青岛266100 3. 中国科学院,海洋研究所,山东,青岛266071 |
| 基金项目： | 国家高技术研究发展计划(863计划) , 青岛市自然科学基金 |
| 摘 要： | 虾青素是一种具有极强抗氧化活性的类胡萝卜素,具有广泛的应用价值。β-胡萝卜素酮化酶(BKT)和β-胡萝卜素羟化酶(CrtR-B)是虾青素的生物合成途径中的两个关键酶。采用LaTaqDNA聚合酶用PCR的方法分别从pET-28a(+)-bkt,pET-28a(+)-CrtR中扩增得到bkt和CrtR-B基因,用bkt基因替换pBI221中的GUS基因形成含有CaMV 35S启动子和NOS终止子的bkt基因表达盒,然后插入植物表达载体pCAMBIA1301的多克隆位点,再用CrtR-B基因替换pCAMBIA1301中的另一个GUS基因,形成CrtR-B基因表达盒,最终获得带有抗性基因的双价植物表达载体pCAMBIA1301-bkt-CrtR。通过农杆菌EHA105介导将其转入花生(花育23),获得了经卡那霉素筛选的抗性植株,通过PCR扩增验证,花生基因组中含有这两个基因。 |
| 关 键 词： | 虾青素 bkt基因 CrtR-B基因 载体构建 花生 |
| 文章编号： | 1004-3268(2007)12-0035-05 |
| 修稿时间： | 2007年6月4日 |
Construction of Plant Expression Vector Containing Genes Encoding Two Key Biosynthesis Enzymes of Astaxanthin( bkt and CrtR-B) and Its Expression in Arachis hypogaea L. |
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| XU Jing,YANG Qing-li,TAN Cong-ping,HOU Yan-hua,YU Shan-lin,QIN Song.Construction of Plant Expression Vector Containing Genes Encoding Two Key Biosynthesis Enzymes of Astaxanthin( bkt and CrtR-B) and Its Expression in Arachis hypogaea L.[J].Journal of Henan Agricultural Sciences,2007(12):35-39. | |
| Authors: | XU Jing YANG Qing-li TAN Cong-ping HOU Yan-hua YU Shan-lin QIN Song |
| Abstract: | Astaxanthin with high antioxidant activity is of great application value.BKT and CrtR-B are two key biosynthesis enzymes of astaxanthin,encoded by bkt gene and CrtR-B gene.Bkt gene expression cassette was constructed by replacing GUS gene in vector pBI221 with bkt gene by PCR method,which contained the CaMV 35S promoter and NOS terminator.Plant expression vector pCAMBIA1301-bkt-CrtR with binary gene was constructed by inserting bkt gene expression cassette into multiple cloning site and replacing GUS gene with CrtR-B gene in vector pCAMBIA1301.Bkt gene and CrtR-B gene were transferred into peanut mediated by Agrobacterium tumefaciens and under the pressure of Kanamycin selection,the kanamycin resistant transformants were achieved.The PCR amplification confirmed that bkt gene and CrtR-B gene had been integrated into the peanut genome successfully. |
| Keywords: | Astaxanthin bkt gene CrtR-B gene Plant expression vector Peanut |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |