| 应用辛酸法-DEAE纤维素吸附过滤法提纯的IgG制备RIA用二抗 |
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| 引用本文： | 崔青山,王志学,马海东.应用辛酸法-DEAE纤维素吸附过滤法提纯的IgG制备RIA用二抗[J].中国兽医学报,1993(2). |
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| 作者姓名： | 崔青山 王志学 马海东 |
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| 作者单位： | 兽医大学军事兽医研究所，兽医大学军事兽医研究所，兰后山丹马场管理局农牧研究所 进修生 |
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| 摘 要： | 用辛酸沉淀法结合DEAE纤维素吸附过滤法,从兔、绵羊、驴和人血清中提取IgG,经PAGE和ID分析,其纯度和免疫活性较好.用提纯的兔IgG和人IgG制备的二抗,抗体效价高(驴抗兔IgG高达1024倍,羊抗人IgG达256倍),特异性好,抗体亲和力强,可供作RIA免疫分离沉淀试剂.
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| 关 键 词： | 辛酸沉淀法 DEAE纤维素吸附过滤法 血清IgG 二抗 |
Purification of IgG Used for Preparing Secondary Antibody in RIA by Caprylic Acid Precipitation and DEAE Cellulose Absorption |
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| Cui Qingshan et al.Purification of IgG Used for Preparing Secondary Antibody in RIA by Caprylic Acid Precipitation and DEAE Cellulose Absorption[J].Chinese Journal of Veterinary Science,1993(2). |
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| Authors: | Cui Qingshan |
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| Institution: | Cui Qingshan et al. The Military Veterinary Institute,Veterinary College of PLA |
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| Abstract: | A simple two-step procedure in IgG purification from sera of human, rabbit,sheep and donkey was described. It was precipitated firstly by caprylic acid and then absorpted byDE-52 cellulose. Both the purity and immunoactivity of the resultant IgG were proved to bebetter by PAGE and ID. It was used to immunize the heterologous animals and secondary anti-bodies with high titer, good specificity and potent affinity were obtained. |
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| Keywords: | caprylic acid precipition DEAE cellulose absorption IgG secondary antibody |
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