| 天麻多糖的提取工艺及含量测定研究 |
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| 引用本文： | 朱洁平,李峰,沈业寿.天麻多糖的提取工艺及含量测定研究[J].安徽农业科学,2012(18):9648-9650. |
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| 作者姓名： | 朱洁平 李峰 沈业寿 |
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| 作者单位： | 1. 皖西卫生职业学院,安徽六安,237005
2. 安徽省中药研究与开发重点实验室,安徽合肥,230039 |
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| 基金项目： | 安徽省高等学校省级自然科学研究项目 |
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| 摘 要： | 目的]对天麻多糖的提取工艺进行研究并测定其各组分中多糖、蛋白质和糖醛酸的含量。方法]用正交试验对天麻多糖的提取工艺进行优选,然后采用苯酚-硫酸法、间羟基联苯法和考马斯亮兰法分别测定天麻多糖及其各组分中总糖、糖醛酸和蛋白质的含量。结果]最佳提取工艺条件为A2B2C3,即:提取温度为70℃,提取时间为2.5 h,提取次数为3次;在此条件下,多糖提取率达13.15%。天麻多糖及其各组分中糖含量分别为65.8%、84.6%和87.3%,蛋白质含量分别为2.9%、0.28%和2.7%,糖醛酸含量分别为29.7%、26.3%和39.6%。结论]该方法简便、快速、准确,灵敏度高,为天麻多糖生产的质量控制提供了依据。
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| 关 键 词： | 天麻 多糖 分光光度法 含量测定 |
Extraction Technology of Polysaccharides from RHIZOMA GASTRODIAE and Its Content Determination |
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| Institution: | ZHU Jie-ping et al(West Anhui Health Vocational College,Liuan,Anhui 237005) |
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| Abstract: | Objective] To establish a convenient and accurate method for the content determination of polysaccharides from RHIZOMA GASTRODIAE.Method] Extraction process of RHIZOMA GASTRODIAE polysaccharides was optimized by orthogonal test.The total saccharide content,uronic acid content and protein content were detected by phenol-sulfuric acid method,m-hydroxydiphenyl method,and Coomassie Brilliant method,respectively.Result] The optimal extraction condition was A2B2C3,that is 70 ℃ extraction temperature,2.5 h extraction time,and 3 extraction times.Under this condition,the extraction rate of polysaccharides reached 13.15%.Saccharide contents in different groups of RHIZOMA GASTRODIAE polysaccharides were 65.8%,84.6% and 87.3%;protein contents were 2.9%,0.28% and 2.7%;uronic acid contents were 29.7%,26.3% and 39.6%.Conclusion] This method was rapid,simple,accurate,and sensitive,and could be used for the quality control of RHIZOMA GASTRODIAE polysaccharides. |
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| Keywords: | RHIZOMA GASTRODIAE Polysaccharides Content determination Spectrophotometry |
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