| 三氯酚对小椎实螺急性毒性作用和过氧化物酶活性的影响 |
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| 引用本文： | 宋志慧,杨鲁娜.三氯酚对小椎实螺急性毒性作用和过氧化物酶活性的影响[J].安徽农业科学,2012,40(20):10439-10441. |
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| 作者姓名： | 宋志慧 杨鲁娜 |
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| 作者单位： | 青岛科技大学环境与安全工程学院,山东青岛,266042;青岛科技大学环境与安全工程学院,山东青岛,266042 |
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| 基金项目： | 山东省社会科学规划项目，山东省高等学校实验技术研究项目 |
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| 摘 要： | 目的]探讨2,3,5-三氯酚和2,3,6-三氯酚对小椎实螺的毒性指标。方法]以小椎实螺为供试生物,采用半静态法对小椎实螺进行驯养和试验,测定2,3,5-TCP和2,3,6-TCP对小椎实螺的半数致死浓度(LC50)、联合毒性作用类型和POD活性的影响。结果]2,3,5-TCP和2,3,6-TCP对小椎实螺的96 h LC50分别为0.525和1.925 mg/L,毒性顺序为:2,3,5-TCP>2,3,6-TCP;2,3,5-TCP和2,3,6-TCP的联合作用类型为协同作用;2,3,5-TCP和2,3,6-TCP暴露1 d胁迫小椎实螺POD为诱导-抑制效应,暴露3和5 d胁迫小椎实螺POD为抑制效应,且随暴露时间的延长,不同酶活性变化显著。结论]为进一步探讨氯酚对腹足动物的毒性机理以及对氯酚化合物污染的早期诊断与生态风险评级提供了理论依据。
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| 关 键 词： | 小椎实螺 2 3 5-三氯酚 6-三氯酚 半数致死浓度 过氧化物酶 |
Effects of 2,3,5-TCP and 2,3,6-TCP on Acute Toxicity and POD Activity of Galba pervia |
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| Institution: | SONG Zhi-hui et al(College of Environment and Safety Engineering,Qingdao University of Science and Technology,Qingdao,Shandong 266042) |
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| Abstract: | Objective] To study the toxic effect of 2,3,5-TCP and 2,3,6-TCP on Galba pervia.Method] The G.pervia was cultured by semi-static method,the LC50 and joint action of 2,3,5-TCP and 2,3,6-TCP to G.pervia as well as the POD activity were discussed.Result] The 96 h-LC50 of 2,3,5-TCP and 2,3,6-TCP were 0.525 mg/L and 1.925 mg/L,respectively.The toxicity order of them was 2,3,5-TCP > 2,3,6-TCP,the joint effect of 2,3,5-TCP and 2,3,6-TCP was synergistic;The POD in G.pervia exposed to 2,3,5-TCP and 2,3,6-TCP stress was both induced and inhibited,while it was inhibited when the G.pervia was exposed to 2,3,5-TCP and 2,3,6-TCP stress for 3 d and 5 d,the enzyme activities were changed significantly with the extending days of exposure.Conclusion] The study provides theoretical support for further discussing the toxic effect of TCP to G.pervia as well as the early diagnosis and risk evaluation of TCP compounds. |
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| Keywords: | Galba pervia 2 3 5-TCP 2 3 6-TCP LC50 POD |
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